Simultaneous fluorescent imaging of Cys/Hcy and GSH from different emission channels
Simultaneous fluorescent imaging of Cys/Hcy and GSH from different emission channels
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来自不同发射通道的 Cys/Hcy 和 GSH 同时荧光成像
DOI：
10.1039/c4sc00838c
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发表时间：
2014-01-01
期刊：
影响因子：
8.4
通讯作者：
Guo, Wei
中科院分区：
文献类型：
--
作者：
Liu, Jing;Sun, Yuan-Qiang;Guo, Wei
利用4-甲氧苯硫酚取代派洛宁染料1作为生物硫醇Cys/Hcy和GSH的反应型荧光探针。由于光诱导电子转移（PET）过程，探针本身是非荧光的。Cys（或Hcy）诱导的取代-重排级联反应和GSH诱导的取代反应与探针的反应导致相应的氨基吡喃和硫代吡喃染料具有不同的物理化学性质，使得Cys/Hcy和GSH能够分别从可见和近红外（NIR）发射通道检测，在纯PB缓冲液中具有相对快的动力学和明显的荧光开启响应。在激光扫描共聚焦显微镜的辅助下，我们还证明探针1可以同时检测B16细胞中的Cys/Hcy和GSH。
A 4-methoxythiophenol-substituted pyronin dye 1 was exploited as reaction-type fluorescent probe for biothiols Cys/Hcy and GSH. The probe itself is nonfluorescent due to the photoinduced electron transfer (PET) process. The Cys (or Hcy)-induced substitution–rearrangement cascade reaction and GSH-induced substitution reaction with the probe lead to the corresponding aminopyronin and thiopyronin dyes with distinct photophysical properties, enabling Cys/Hcy and GSH to be detected from visible and near-infrared (NIR) emission channels, respectively, in pure PB buffer with relatively fast kinetics and obvious fluorescence turn-on response. Assisted by laser scanning confocal microscope, we also demonstrated that probe 1 could simultaneously sense Cys/Hcy and GSH in B16 cells in multicolor imaging.