Chronic Omega-3 Polyunsaturated Fatty Acid Treatment Variably Affects Cellular Repolarization in a Healed Post-MI Arrhythmia Model.
Chronic Omega-3 Polyunsaturated Fatty Acid Treatment Variably Affects Cellular Repolarization in a Healed Post-MI Arrhythmia Model.
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DOI：
10.3389/fphys.2016.00225
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发表时间：
2016
影响因子：
4
通讯作者：
Carnes CA
中科院分区：
文献类型：
--
作者：
Bonilla IM;Nishijima Y;Vargas-Pinto P;Baine SH;Sridhar A;Li C;Billman GE;Carnes CA
简介：在过去的40年中，ω-3多不饱和脂肪酸（PUFA）已被证明是抗糖尿病或促糖尿病的，这取决于给药的方法和持续时间以及研究的模型。我们以前报道过，ω-3多不饱和脂肪酸不赋予抗心律失常特性，并在犬心脏性猝死（SCD）模型中具有促心律失常作用。在这里，我们评估了慢性omega-3 PUFA治疗对心肌梗死后室性快速性心律失常敏感（VF+）或抵抗（VF-）的动物的影响。研究方法：采用穿孔膜片钳技术测定心肌细胞复极50%和90%时的动作电位时程（APD）及复极短期变异性。并对早期复极化瞬时外向钾电流Ito进行了研究。结果：Omega-3 PUFA延长了VF−肌细胞在50%和90%复极化时的动作电位。与对照组相比，未处理和处理的VF−肌细胞复极的短期变异性增加。Ito不受omega-3 PUFA治疗的影响。Omega-3 PUFA处理减弱VF+肌细胞的动作电位延长，但未使复极恢复至对照值。结论：在SCD犬模型中，Omega-3 PUFAs在愈合的心肌梗死的情况下不具有抗心律失常特性。在先前对室颤（VF−）有抵抗力的犬中，omega-3 PUFA治疗延长了VF−肌细胞的动作电位，并可能有助于促心律失常反应。
Introduction: Over the last 40 years omega-3 polyunsaturated fatty acids (PUFAs) have been shown to be anti-arrhythmic or pro-arrhythmic depending on the method and duration of administration and model studied. We previously reported that omega-3 PUFAs do not confer anti-arrhythmic properties and are pro-arrhythmic in canine model of sudden cardiac death (SCD). Here, we evaluated the effects of chronic omega-3 PUFA treatment in post-MI animals susceptible (VF+) or resistant (VF−) to ventricular tachyarrhythmias. Methods: Perforated patch clamp techniques were used to measure cardiomyocyte action potential durations (APD) at 50 and 90% repolarization and short term variability of repolarization. The early repolarizing transient outward potassium current Ito was also studied. Results: Omega-3 PUFAs prolonged the action potential in VF− myocytes at both 50 and 90% repolarization. Short term variability of repolarization was increased in both untreated and treated VF− myocytes vs. controls. Ito was unaffected by omega-3 PUFA treatment. Omega-3 PUFA treatment attenuated the action potential prolongation in VF+ myocytes, but did not return repolarization to control values. Conclusions: Omega-3 PUFAs do not confer anti-arrhythmic properties in the setting of healed myocardial infarction in a canine model of SCD. In canines previously resistant to ventricular fibrillation (VF−), omega-3 PUFA treatment prolonged the action potential in VF− myocytes, and may contribute to pro-arrhythmic responses.