Conversion of the ω subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target
Conversion of the ω subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target
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DOI：
10.1101/gad.12.5.745
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发表时间：
1998-03-01
影响因子：
10.5
通讯作者：
Hochschild, A
中科院分区：
文献类型：
--
作者：
Dove, SL;Hochschild, A
在真核生物和原核生物中获得的证据表明，DNA结合的蛋白质和转录机制的组分之间的任意接触可以激活转录。在这里，我们表明，大肠杆菌欧米茄蛋白，它与RNA聚合酶copurifies，可以作为一个转录激活剂时，共价连接到一个DNA结合蛋白。我们进一步表明，欧米茄可以作为一个激活目标时，这种共价键被替换为一对相互作用的多肽融合的DNA结合蛋白和欧米茄，分别。我们的研究结果表明，ω蛋白与RNA聚合酶全酶在体内，并提供支持的假设，DNA结合的蛋白质之间的接触和任何组成部分的E。coli RNA聚合酶可以激活转录。
Evidence obtained in both eukaryotes and prokaryotes indicates that arbitrary contacts between DNA-bound proteins and components of the transcriptional machinery can activate transcription. Here we demonstrate that the Escherichia coli omega protein, which copurifies with RNA polymerase, can function as a transcriptional activator when linked covalently to a DNA-binding protein. We show further that omega can function as an activation target when this covalent linkage is replaced by a pair of interacting polypeptides fused to the DNA-binding protein and to omega, respectively. Our findings imply that the omega protein is associated with RNA polymerase holoenzyme in vivo, and provide support for the hypothesis that contact between a DNA-bound protein and any component of E. coli RNA polymerase can activate transcription.