Development and heat stress-induced transcriptomic changes during embryogenesis of the scleractinian coral Acropora palmata
Development and heat stress-induced transcriptomic changes during embryogenesis of the scleractinian coral Acropora palmata
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DOI：
10.1016/j.margen.2010.03.002
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发表时间：
2010-03-01
期刊：
影响因子：
1.9
通讯作者：
Szmant, Alina M.
中科院分区：
文献类型：
--
作者：
Portune, Kevin J.;Voolstra, Christian R.;Szmant, Alina M.
预计海水温度将升高，这将威胁到加勒比造礁珊瑚的繁殖成功，这些珊瑚在一年中最温暖的月份里进行浮游式发育。本研究探讨了在胚胎和幼虫发育过程中发生的埃尔克霍恩珊瑚，鹿角珊瑚，在非应激温度（28摄氏度）的转录组的变化，并进一步评估了两个升高的温度（30摄氏度和31.5摄氏度）对这些表达模式的影响。使用cDNA微阵列，我们比较了2051个基因的表达水平，从早期胚胎和幼虫在多个发育阶段（包括前囊胚，囊胚，原肠胚，和planula阶段）在每三个温度。在28 ℃处理的受精后12 h，与细胞复制/细胞分裂和转录相关的基因在A.掌叶胚胎，其次是这些基因的表达在以后的生长阶段减少。在28 ℃条件下，受精后24.5 ~ 131 h，A. palmata通过上调参与蛋白质合成和代谢的基因来改变其转录组。30摄氏度和31.5摄氏度的温度引起了A的重大变化。palmata胚胎转录组，特别是在受精后24.5 hpf的样品中，其特征在于与28 ℃处理相比，参与细胞复制/细胞分裂、代谢、细胞骨架和转录的许多基因下调，而热休克基因上调。这些结果表明，温度升高可能会导致在适当的基因表达在发育过程中，在A。通过下调参与基本细胞过程的基因来抑制掌状细胞，这可能会导致其他研究中记录的异常发育和生存率降低。(C)2010 Elsevier B. V.保留所有权利。
Projected elevation of seawater temperatures poses a threat to the reproductive success of Caribbean reef-building corals that have planktonic development during the warmest months of the year. This study examined the transcriptomic changes that occurred during embryonic and larval development of the elkhorn coral, Acropora palmata, at a non-stressful temperature (28 degrees C) and further assessed the effects of two elevated temperatures (30 degrees C and 31.5 degrees C) on these expression patterns. Using cDNA microarrays, we compared expression levels of 2051 genes from early embryos and larvae at multiple developmental stages ( including pre-blastula, blastula, gastrula, and planula stages) at each of the three temperatures. At 12 h post-fertilization in 28 degrees C treatments, genes involved in cell replication/cell division and transcription were up-regulated in A. palmata embryos, followed by a reduction in expression of these genes during later growth stages. From 24.5 to 131 h post-fertilization at 28 degrees C, A. palmata altered its transcriptome by up-regulating genes involved in protein synthesis and metabolism. Temperatures of 30 degrees C and 31.5 degrees C caused major changes to the A. palmata embryonic transcriptomes, particularly in the samples from 24.5 hpf post-fertilization, characterized by down-regulation of numerous genes involved in cell replication/cell division, metabolism, cytoskeleton, and transcription, while heat shock genes were up-regulated compared to 28 degrees C treatments. These results suggest that increased temperature may cause a breakdown in proper gene expression during development in A. palmata by down-regulation of genes involved in essential cellular processes, which may lead to the abnormal development and reduced survivorship documented in other studies. (C) 2010 Elsevier B.V. All rights reserved.