mRNA for cardiac calcium channel is expressed during development of skeletal muscle.
mRNA for cardiac calcium channel is expressed during development of skeletal muscle.
复制标题
心脏钙通道的 mRNA 在骨骼肌发育过程中表达。
DOI：
10.1006/dbio.1993.1048
复制
发表时间：
1993
影响因子：
2.7
通讯作者：
Beam,KG
中科院分区：
文献类型：
--
作者：
Chaudhari,N;Beam,KG
在骨骼肌的早期发育过程中，已知几种收缩蛋白的心脏同种型瞬时表达。我们在这里报告，骨骼肌developingin体内，以及来自骨骼肌的原代培养物，表达编码心脏二氢吡啶敏感性钙通道的mRNA。mRNA在体内测试的最早阶段以高浓度检测，并且随着肌纤维成熟而浓度迅速减少。在遗传性瘫痪小鼠（mdg）的骨骼肌体内发育过程中，心脏钙通道mRNA的浓度也减少，表明肌肉收缩活性不是下调所必需的。相反，骨骼肌特异性钙通道的mRNA在发育中的骨骼肌中逐渐积累。在骨骼肌管的原代培养物中也观察到类似的表达时间模式。这些结果提出了一个问题，是否心脏钙通道可能在骨骼肌纤维的早期发育过程中的功能重要。
During the early development of skeletal muscle, cardiac isotypes of several contractile proteins are known to be transiently expressed. We report here that skeletal muscle developingin vivo, as well as primary cultures derived from skeletal muscle, express mRNA encoding the cardiac dihydropyridine-sensitive calcium channel. The mRNA is detectable at high concentration at the earliest stage testedin vivoand diminishes rapidly in concentration as myofibers mature. The concentration of the cardiac calcium channel mRNA also diminishes during thein vivodevelopment of skeletal muscle in a genetically paralyzed mouse (mdg), indicating that muscle contractile activity is not necessary for the down-regulation. In contrast, mRNA for the skeletal muscle-specific calcium channel accumulates gradually in developing skeletal muscle. A similar temporal pattern of expression is also seen in primary cultures of skeletal myotubes. These results raise the question of whether the cardiac calcium channel may be functionally important during the early development of skeletal myofibers.