Isolation and characterization of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros sp. (strain TG07-C28)
Isolation and characterization of a single-stranded DNA virus infecting the marine planktonic diatom Chaetoceros sp. (strain TG07-C28)
复制标题
感染海洋浮游硅藻角毛藻的单链 DNA 病毒的分离和表征。
DOI：
10.3800/pbr.7.20
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发表时间：
2012
影响因子：
0.6
通讯作者：
Y. Tomaru
中科院分区：
文献类型：
--
作者：
Kensuke Toyoda;K. Kimura;N. Hata;N. Nakayama;Keizo Nagasaki;Y. Tomaru
海洋中硅藻的动态是海洋生态学家最感兴趣的研究课题之一。最近的研究表明，病毒是硅藻种群死亡的潜在来源，以及更传统的环境因素。在这里，我们报告了一种新的单链DNA（ssDNA）病毒（Csp 05 DNAV）的分离和表征，该病毒导致角毛藻TG 07-C28的裂解，分离自日本阿戈湾的地表水。该病毒是从阿戈湾的沉积物中分离出来的，其感染既具有裂解性又具有物种特异性。估计潜伏期<24 h。病毒粒子直径为32-34 nm，聚集于宿主细胞核内。Csp 05 DNAV具有闭合环状ssDNA基因组，其包括部分双链区域。部分序列分析显示，该病毒基因组的开放阅读框与先前报道的携带ssDNA基因组的硅藻病毒的推定复制酶相关蛋白相似;特异性DNA、CsalDNAV、CdebDNAV、ClorDNAV和CtenDNAV。根据基因组结构，Csp 05 DNAV属于杆状病毒属。由于宿主和病毒都是从Ago湾分离到的，因此宿主-病毒系统可能在该湾具有重要的生态意义。这一发现为阐明沿海沃茨沉积动力学提供了基础信息。
Diatom dynamics in the ocean represents one of the most interesting research themes for marine ecolo- gists. Recent studies have shown the significance of viruses as potential sources of mortality for diatom populations, as well as the more traditional environmental factors. Here, we report the isolation and characterization of a new sin- gle-stranded DNA (ssDNA) virus (Csp05DNAV) that causes lysis in Chaetoceros sp. TG07-C28, isolated from the surface water of Ago Bay, Japan. The virus was isolated from the sediments in Ago Bay, and its infection was both lytic and species-specific. The latent period was estimated to be <24 h. The virion is 32-34 nm in diameter, and ac- cumulates in the nucleus of host cells. Csp05DNAV has a closed circular ssDNA genome, which includes a partially double-stranded region. Partial sequence analysis revealed that the open reading frame of this virus genome was sim- ilar to the putative replicase-related proteins of previously reported diatom viruses that harbor a ssDNA genome; spe- cifically, CsalDNAV, CdebDNAV, ClorDNAV, and CtenDNAV. Based on the genome structure, Csp05DNAV is con- sidered to belong to the genus Bacilladnavirus. As both the host and virus were isolated from Ago Bay, the host-virus system probably is ecologically important in the bay. This finding provides basic information towards elucidating dia- tom dynamics in coastal waters.