Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment
Parasulfuritortus cantonensis gen. nov., sp. nov., a microaerophilic sulfur-oxidizing bacterium isolated from freshwater sediment
复制标题
Parasulfuritortus cantonensis gen.
DOI：
10.1099/ijsem.0.004657
复制
发表时间：
2021
影响因子：
2.8
通讯作者：
Jianfei Luo
中科院分区：
文献类型：
--
作者：
Zhangzhang Xie;Surong Li;Weitie Lin;Jianfei Luo
从广州珠江淡水沉积物中分离到一株新的硫氧化细菌，命名为LSR1T，命名为PR中国。该菌株是一种专性化学自养细菌，使用硫代硫酸盐或硫化物作为电子供体和能源。菌株LSR1T在15-40℃、pH 6.0-7.5和0-1.5%的氯化钠浓度下生长。菌株LSR1T具有微嗜氧性，在含氧量低于10%时才能生长。当硝酸盐作为唯一的电子受体时，也观察到厌氧生长。主要的细胞脂肪酸是C16：0和特征3(包括C16：1ω7c和/或C16：1ω6c)。测得草鱼基因组DNA G+C含量为67.5mol%。基于16S rRNA基因序列的系统发育分析表明，菌株LSR1T在该家族中形成了一个谱系。硫杆菌科。与其近缘属的序列同源性分别为92.87%、92.33%和90.80%。硫磺。、。安伍迪亚。而且.。硫杆菌。，分别为。LSR1T菌株基因组包含多个编码硫代硫酸盐和硫化物氧化的硫氧化酶基因和编码CBB的基因。3型细胞色素C氧化酶和BD型喹酚氧化酶，使菌株LSR1T能够在微好氧条件下进行硫氧化。根据表型、基因分型和系统发育结果，菌株LSR1T被认为是该科一新属的新种。硫杆菌科。，命名为广州拟硫磺藻ParasulFuritortus cantonensis Gen.Nov.，sp.11月是提议的。模式菌株为LSR1T(=GDMCC 33645=1.1549)。
A novel sulfur-oxidizing bacterium, designated strain LSR1T, was enriched and isolated from a freshwater sediment sample collected from the Pearl River in Guangzhou, PR China. The strain was an obligate chemolithoautotroph, using thiosulfate or sulfide as an electron donor and energy source. Growth of strain LSR1T was observed at 15–40 °C, pH 6.0–7.5 and NaCl concentrations of 0–1.5 %. Strain LSR1T was microaerophilic, with growth only at oxygen content less than 10 %. Anaerobic growth was also observed when using nitrate as the sole electron acceptor. The major cellular fatty acids were C16 : 0 and summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c). The DNA G+C content of the draft genome sequence was 67.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LSR1T formed a lineage within the family . Thiobacillaceae. , showing sequence identities of 92.87, 92.33 and 90.80 % with its closest relative genera . Sulfuritortus. , . Annwoodia. and . Thiobacillus. , respectively. The genome of strain LSR1T contained multiple genes encoding sulfur-oxidizing enzymes that catalyse thiosulfate and sulfide oxidation, and the gene encoding cbb. 3-type cytochrome c oxidase and bd-type quinol oxidase, which enables strain LSR1T to perform sulphur oxidation under microaerophilic conditions. On the basis of phenotypic, genotypic and phylogenetic results, strain LSR1T is considered to represent a novel species of a new genus Parasulfuritortus within the family . Thiobacillaceae. , for which the name Parasulfuritortus cantonensis gen. nov., sp. nov. is proposed. The type strain is LSR1T (=GDMCC 1.1549=JCM 33645).