Genome Editing in Mouse and Rat by Electroporation
Genome Editing in Mouse and Rat by Electroporation
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通过电穿孔对小鼠和大鼠进行基因组编辑
DOI：
10.1007/978-1-0716-3016-7_10
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发表时间：
2023
影响因子：
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通讯作者：
Takehitio Kaneko
中科院分区：
文献类型：
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作者：
Kaku Yu;Matsumoto Kaho;Kuwata Takeo;Zahid Hasan Md;Biswas Shashwata;Gorny Miroslaw K.;Matsushita Shuzo;Takehitio Kaneko
许多基因组编辑的小鼠和大鼠品系已经使用工程化核酸内切酶产生，包括锌指核酸酶（ZFN）、转录激活因子样效应物核酸酶（TALEN）或成簇规则间隔短回文重复序列（CRISPR）-Cas9。特别是，CRISPR-Cas9是一种强大的工具，可以简单，快速，高效地生产新的基因组编辑菌株。此外，新技术，电穿孔动物敲除系统技术（TAKE），通过使用电穿孔将核酸酶直接引入完整胚胎中，有效地加速了新菌株的产生。本章介绍了通过TAKE方法生产基因组编辑小鼠和大鼠的最新技术信息。
Many genome-edited mouse and rat strains have been produced using engineered endonucleases, including zinc finger nuclease (ZFN),transcription activator-like effector nuclease(TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Especially, CRISPR-Cas9 is powerful tool that can be easy, rapid, and high-efficiency-produced new genome-edited strains. Furthermore, new technique, Technique for Animal Knockout system by Electroporation (TAKE), efficiently accelerate production of new strains by direct nuclease introduction into intact embryos using electroporation. This chapter presents a latest technical information in the production of genome-edited mouse and rat by TAKE method.