Can oxygen tension contribute to an abnormal placental cytokine milieu?
Can oxygen tension contribute to an abnormal placental cytokine milieu?
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氧张力会导致胎盘细胞因子环境异常吗？
DOI：
10.1111/j.1600-0897.2011.00998.x
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Hanna,Nazeeh
中科院分区：
文献类型：
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作者：
Peltier,MorganR;Gurzenda,EllenM;Murthy,Amitasrigowri;Chawala,Kiranpreet;Lerner,Veronica;Kharode,Ishita;Arita,Yuko;Rhodes,Adam;Maari,Nisreen;Moawad,Andrew;Hanna,Nazeeh
引用Peltier MR，Gurzenda EM，Murthy A，Chawala K，Lerner V，Kharode I，Arita Y，罗兹A，Maari N，Moawad A，Hanna N.氧分压能导致异常的胎盘细胞因子环境吗？Am J Reprod Immunol 2011; 66：279- 285本研究的目的是确定在不同氧分压下培养人胎盘外植体是否会改变促炎和抗炎介质的表达。MethodsPlacental explant cultures from middle-trimester，elective，terminations-of-pregnancy were incubated under 21，5，or 1% O2 concentrations for 24 hr in the presence or absence of IL-10. 结果胎盘组织培养在21、5或1%O2浓度下，母胎界面的pO 2水平分别为高氧（143± 1.6 mmHg）、常氧（37 ± 1.6 mmHg）和低氧（18.2 ± 1.6 mmHg）。 氧分压对基础胎盘细胞因子水平以及IL-10刺激的细胞因子产生有深远影响。IL-1β和TNF-α，但不是IFN-γ的产生，减少了21%的O2。此外，21%O2水平增加了抗炎细胞因子IL-10和IL-13，而1%O2往往会减少这些cytokines.ConclusionsFive百分之O2孵育更准确地代表在vivopO 2条件下的母胎界面。在室内空气中对胎盘外植体进行常规培养会产生超生理氧张力，这往往会增加抗炎因子的产生并减少促炎细胞因子的产生。此外，低pO 2可能会降低胎盘对IL-10抗炎作用的反应性。
CitationPeltier MR, Gurzenda EM, Murthy A, Chawala K, Lerner V, Kharode I, Arita Y, Rhodes A, Maari N, Moawad A, Hanna N. Can oxygen tension contribute to an abnormal placental cytokine milieu? Am J Reprod Immunol 2011; 66: 279–285ObjectiveThe aim of this study was to determine whether culturing human placental explants under different oxygen tensions will alter expression of pro‐ and anti‐inflammatory mediators.MethodsPlacental explant cultures from second‐trimester, elective, terminations‐of‐pregnancy were incubated under 21, 5, or 1% O2concentrations for 24 hr in the presence or absence of IL‐10. Cytokine concentrations in the conditioned medium were quantified by immunoassay.ResultsCulture of placental explants under 21, 5, or 1% O2concentrations produced hyperoxic (143 ± 1.6 mmHg), normoxic (37 ± 1.6 mmHg), and hypoxic (18.2 ± 1.6 mmHg) pO2levels for the maternal–fetal interface in the medium. Oxygen tension had profound effects on basal placental cytokine levels as well as on IL‐10‐stimulated cytokine production. IL‐1β and TNF‐α, but not IFN‐γ production, was reduced by 21% O2. Moreover, 21% O2levels increased the anti‐inflammatory cytokines IL‐10 and IL‐13 while 1% O2tended to decrease the production of these cytokines.ConclusionsFive percent‐ O2incubation more accurately representsin vivopO2conditions at the maternal–fetal interface. Routine culture of placental explants in room air produces a superphysiologic oxygen tension that tended to increase the production of anti‐inflammatory and decrease the production of pro‐inflammatory cytokines. In addition, low pO2may reduce responsiveness of the placenta to the anti‐inflammatory actions of IL‐10.