Osmolar changes regulate the paracellular permeability of cultured human cervical epithelium.
Osmolar changes regulate the paracellular permeability of cultured human cervical epithelium.
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渗透压变化调节培养的人宫颈上皮的细胞旁通透性。
DOI：
10.1152/ajpcell.1995.269.4.c870
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hopfer,U
中科院分区：
文献类型：
--
作者：
Gorodeski,GI;DeSantis,BJ;Goldfarb,J;Utian,WH;Hopfer,U
细胞外核苷酸诱导过滤器上生长的人宫颈细胞的跨上皮电导（GT）的双相变化：快速增加（I相），然后持续下降（II相）。为了探测细胞间隙的参与，通过改变细胞外渗透压来操纵细胞体积来改变其大小。在基线条件下[GT = 115 mS/cm 2（约9 Ω. cm 2）]和在阶段II期间，高渗激发导致GT增加（分别为0.98%.mosmol-1.l-1和0.73%.mosmol-1.l-1）。然而，在I期期间的高渗激发降低了GT（-0.16%.mosmol-1.l-1）。在基线、I期和II期条件下，低渗激发使GT降低约1%. mosmol-1.l-1。观察到类似的趋势与吡喃渗透性。减少细胞外钙增加GT，废除细胞外ATP的II相效应，并逆转高渗挑战的效果。在II期期间响应于渗透挑战和ATP的渗透性变化的加和性质表明，不同的位点参与每个响应，即，细胞间隙的电阻随渗透压和紧密连接的渗透压而变化。
Extracellular nucleotides induce a biphasic change in the transepithelial electrical conductance (GT) of human cervical cells grown on filters: a rapid increase (phase I) followed by a sustained decrease (phase II). To probe the involvement of the intercellular space, its magnitude was varied by manipulating cell volume through changes in extracellular osmolarity. Under baseline conditions [GT = 115 mS/cm2 (approximately 9 omega.cm2)] and during phase II, hypertonic challenges resulted in an increase in GT (0.98% .mosmol-1.l-1 and 0.73%.mosmol-1.l-1, respectively). However, a hypertonic challenge during phase I decreased GT (-0.16%.mosmol-1.l-1). Hypotonic challenges decreased GT during baseline, phase I, and phase II conditions by -1%.mosmol-1.l-1. Similar trends were observed with regard to pyranine permeability. Reduction of extracellular calcium increased GT, abrogated the phase II effect of extracellular ATP, and reversed the effect of a hypertonic challenge. The additive nature of the permeability changes in response to osmotic challenges and to ATP during phase II suggests that different sites are involved in each response, i.e., the resistance of the intercellular space changes with osmolarity and that of the tight junction during phase II.