Matrix metalloproteinases in blood from patients with LAM
Matrix metalloproteinases in blood from patients with LAM
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DOI：
10.1016/j.rmed.2008.07.017
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Nishimura, Masaharu
中科院分区：
文献类型：
--
作者：
Odajima, Nao;Betsuyaku, Tomoko;Nishimura, Masaharu
肺淋巴管平滑肌瘤病（LAM）以异常平滑肌细胞（LAM细胞）增殖和肺泡结构破坏为特征。免疫组化研究表明，过量的基质金属蛋白酶（MMPs）合成的LAM细胞功能的蛋白水解机制，这种疾病。我们推测LAM患者血液中MMP水平升高。从36例LAM患者和25例对照中收集血清样品，并通过明胶酶谱法半定量明胶分解活性。MMP-9血清数据的可靠性通过酶联免疫吸附测定法以及明胶酶谱法测定血浆中MMP-9浓度来证实。与对照组（0.1 +/- 0 Au）相比，LAM患者的血清MMP-9水平（0.7 +/- 0.1 Au）显著升高，但MMP-2水平没有显著升高。血浆和血清MMP-9水平显著相关。这些结果表明MMP-9参与LAM。(C)2008爱思唯尔有限公司保留所有权利。
Pulmonary lymphangioleiomyomatosis (LAM) is characterized by the proliferation of abnormal smooth muscle cells (LAM cells) and destruction of alveolar structure. Immunohistochemical studies suggest that excess matrix metalloproteinases (MMPs) synthesized by LAM cells function in the proteolytic mechanisms of this disease. We postulated MMP levels in the blood are elevated in LAM patients. Serum samples were collected from 36 LAM patients and 25 controls and gelatinolytic activities were semi-quantified by gelatin zymography. The reliability of serum data for MMP-9 was confirmed by the measurement of MMP-9 concentration in plasma by enzyme-linked immunosorbent assay as well as by gelatin zymography. Serum levels of MMP-9 (0.7 +/- 0.1 AU), but not MMP-2, were significantly elevated in LAM patients compared with controls (0.1 +/- 0 AU). Plasma and serum levels of MMP-9 significantly correlated. These results suggest the involvement of MMP-9 in LAM. (C) 2008 Elsevier Ltd. All rights reserved.