Transcriptional profiling of Mycoplasma hyopneumoniae during heat shock using microarrays
Transcriptional profiling of Mycoplasma hyopneumoniae during heat shock using microarrays
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DOI：
10.1128/iai.74.1.160-166.2006
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发表时间：
2006-01-01
影响因子：
3.1
通讯作者：
Minion, FC
中科院分区：
文献类型：
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作者：
Madsen, ML;Nettleton, D;Minion, FC
细菌病原体在宿主定殖和疾病过程中经历应激。这些压力导致基因表达的变化，以补偿疾病期间宿主中产生的潜在致命环境。猪肺炎支原体定植于猪上皮细胞，引起肺炎，使宿主易受其他病原体的感染。如何M。目前尚不清楚在疾病进展过程中，甲低对呼吸道环境变化的反应。事实上，关于支原体对变化的生长环境的反应能力知之甚少。由于基因有限，支原体被认为只有少数基因调控机制。微阵列由M的698个开放阅读框中的632个组成。构建了一个基因表达谱，并用于研究从37摄氏度到42摄氏度的温度变化期间的基因表达差异，这是疾病期间可能遇到的温度波动。为了提高灵敏度，采用独特的六聚体引物组仅从mRNA种类产生cDNA。我们的分析确定了91个基因在对节拍休克条件的反应中有显著的转录差异（P
Bacterial pathogens undergo stress during host colonization and disease processes. These stresses result in changes in gene expression to compensate for potentially lethal environments developed in the host during disease. Mycoplasma hyopneumoniae colonizes the swine epithelium and causes a pneumonia that predisposes the host to enhanced disease from other pathogens. How M. hyopneumoniae responds to changing environments in the respiratory tract during disease progression is not known. In fact, little is known concerning the capabilities of mycoplasmas to respond to changing growth environments. With limited genes, mycoplasmas are thought to possess only a few mechanisms for gene regulation. A microarray consisting of 632 of the 698 open reading frames of M. hyopneumoniae was constructed and used to study gene expression differences during a temperature shift from 37 degrees C to 42 degrees C, a temperature swing that might be encountered during disease. To enhance sensitivity, a unique hexamer primer set was employed for generating cDNA from only mRNA species. Our analysis identified 91 genes that had significant transcriptional differences in response to beat shock conditions (P