Anaerobic Bacteria on the Mucosal Epithelium of the Murine Large Bowel
Anaerobic Bacteria on the Mucosal Epithelium of the Murine Large Bowel
复制标题
小鼠大肠粘膜上皮上的厌氧细菌
作者：
D. Savage;J. Mcallister;C. P. Davis
在美国分布广泛的四个不同菌落的成年小鼠中，每克盲肠或结肠中可检测到1011个厌氧菌。这些微生物大多数是不耐氧的梭形细菌。在小鼠大肠的冷冻切片组织学准备中，至少可以在上皮黏液层中看到一种锥形棒状细菌。此外，在电镜检查的结肠或盲肠超薄切片中，这些微生物在上皮0.5 μm内可见。这些梭形细菌在黏液层中占优势。然而，在结肠超薄切片的粘膜上皮附近也可以发现螺旋形微生物。此外，在电子显微镜下检查的结肠粘膜上皮冲洗物的阴性染色制备中也可以看到这些微生物。至少有三种螺旋形生物，包括螺旋形细菌和螺旋体，可以在来自四个菌落中的三个菌落的小鼠制剂中发现。在所有四个菌落的小鼠大肠的厌氧培养中，可以检测到这种螺旋形微生物的种群水平高达每克盲肠或结肠109个微生物。在纯培养中分离到1株厌氧螺旋菌。免疫荧光法发现这种特殊的微生物与小鼠大肠粘膜上皮黏液中的梭形细菌混合在一起。
Anaerobic bacteria can be detected at population levels of 1011 organisms per g of cecum or colon in adult mice from four different colonies widely spaced in the United States. Most of these microorganisms are oxygen-intolerant fusiform-shaped bacteria. At least one type of these tapered, rod-shaped bacteria can be seen in layers in the epithelial mucin in frozen-section histological preparations of the large bowels of mice. In addition, such microorganisms can be seen within 0.5 μm of the epithelium in ultrathin sections of colon or cecum examined in an electron microscope. These fusiform-shaped bacteria predominate in the mucin layers. However, spiral-shaped microorganisms can be found as well near the mucosal epithelia in ultrathin sections of colon. Also, such organisms can be seen in negatively-stained preparations of washings of the colonic mucosal epithelia examined in an electron microscope. At least three types of spiral-shaped organisms, including both spiral-shaped bacteria and spirochetes, can be found in preparations from mice from three of the four colonies. Such spiral-shaped microorganisms can be detected at population levels as great as 109 organisms per g of cecum or colon in anaerobic cultures of the large bowels of mice from all four colonies. One anaerobic spiral bacterium was isolated in pure culture. This particular organism was found by immunofluorescence to be intermingled with the fusiform-shaped bacteria in the mucin on the mucosal epithelium in the mouse large bowel.