Lactobacillus plantarum ZJUIDS14 alleviates non-alcoholic fatty liver disease in mice in association with modulation in the gut microbiota.
Lactobacillus plantarum ZJUIDS14 alleviates non-alcoholic fatty liver disease in mice in association with modulation in the gut microbiota.
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植物乳杆菌ZJUIDS 14在小鼠中与肠道微生物群的调节相关的非酒精性脂肪肝疾病。
DOI：
10.3389/fnut.2022.1071284
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发表时间：
2022
影响因子：
5
通讯作者：
Ren, Daxi
中科院分区：
文献类型：
--
作者：
Cao, Feiwei;Ding, Qinchao;Zhuge, Hui;Lai, Shanglei;Chang, Kaixin;Le, Chunyan;Yang, Guorong;Valencak, Teresa G.;Li, Songtao;Ren, Daxi
关键词：
本研究旨在探讨植物乳杆菌ZJUIDS14对高脂饮食诱导的C57BL/6小鼠非酒精性脂肪性肝病(NAFLD)的保护作用。益生菌(109CFU/次，隔日一次)灌胃给药12周。我们发现，植物乳杆菌ZJUIDS14干预显著减轻了高脂血症所致的肝脂肪变性、肝损伤、胰岛素抵抗，并增加了肝组织过氧化体增殖物激活受体α(PPAR-α)的表达，同时刺激了AMP激活的蛋白激酶(AMPK)的激活。此外，植物乳杆菌ZJUIDS14改善了线粒体功能，反映在处理后动力蛋白相关蛋白1(Drp1)的增加和氧化磷酸化相关蛋白(OXPHOS)的减少。此外，来自植物乳杆菌ZJUIDS14组的小鼠的肠道菌群和动态平衡得到了恢复，包括Coprostanenes组、反刍球菌科UCG-014组、Allobaculum、Rumiclostridium 1和Roseburia。同时，Spearman相关分析显示，这5个属与回肠炎症mRNA水平和SCFA含量呈显著(负或正)相关。总体而言，我们的数据表明，植物乳杆菌ZJUIDS14可以减轻HFD引起的肝脏脂肪变性和肝损伤。具体地说，它们加强了肠道屏障的完整性，调节了肠道微生物区系，并改善了线粒体功能。我们的研究结果为植物乳杆菌ZJUIDS14作为预防非酒精性脂肪性肝病的候选菌株提供了实验依据。
This present study was designed to explore the protective role of Lactobacillus plantarum ZJUIDS14 against Non-alcoholic Fatty Liver Disease (NAFLD) in a high-fat-diet (HFD)-induced C57BL/6 mice model. The probiotic (109 CFU/every other day) was administered by oral gavage for 12 weeks. We found that L. plantarum ZJUIDS14 intervention significantly alleviated HFD related hepatic steatosis, liver damage, insulin resistance, and increased hepatic expression of peroxisome proliferator activated receptor α (PPAR-α) while stimulating the activation of AMP-activated protein kinase (AMPK). Furthermore, L. plantarum ZJUIDS14 improved mitochondrial function as reflected by an increase in dynamin related protein 1 (DRP1) and a decrease of proteins associated with oxidative phosphorylation (OXPHOS) after the treatment. Additionally, mice from the L. plantarum ZJUIDS14 group had a restored intestinal flora and homeostasis involving Coprostanoligenes group, Ruminococcaceae UCG-014, Allobaculum, Ruminiclostridium 1, and Roseburia. Meanwhile, these five genera exhibited a significant (negative or positive) association with ileum inflammation mRNA levels and SCFA contents, by Spearman’s correlation analysis. In general, our data demonstrated that L. plantarum ZJUIDS14 mitigates hepatic steatosis and liver damage induced by HFD. Specifically, they strengthened the integrity of the intestinal barrier, regulated gut microbiota, and improved mitochondrial function. Our data provide an experimental basis for L. plantarum ZJUIDS14 as a promising candidate to prevent NAFLD.
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影响因子：
82.9
作者：
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通讯作者：
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