SEXUALITY AND CYST FORMATION IN THE DINOFLAGELLATE GONYAULAX-TAMARENSIS - CYST YIELD IN BATCH CULTURES
SEXUALITY AND CYST FORMATION IN THE DINOFLAGELLATE GONYAULAX-TAMARENSIS - CYST YIELD IN BATCH CULTURES
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DOI：
10.1111/j.0022-3646.1984.00418.x
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
BINDER, BJ
中科院分区：
文献类型：
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作者：
ANDERSON, DM;KULIS, DM;BINDER, BJ
对有毒甲藻G. tamarensis Leoptera（var.在暴露于多种营养和环境处理的分批培养物中监测了凹口藻（incubataL.）氮（如铵或硝酸盐）或磷（如磷酸盐）的限制导致包囊形成。当限制营养的初始浓度是不同的，总包囊产量（ml-1）是成正比的细胞产量，但在所有的最高营养浓度（其中包囊是最小的）。在5倍的细胞密度范围内，包囊效率相对恒定（0.1-0.2个包囊/细胞），表明20-40%的营养群体成功包囊。在营养丰富的培养基中，即使由于非最佳光照、温度或高分批培养细胞密度而导致生长速率显著降低，囊肿形成也可以忽略不计。低光水平降低包囊产量一旦包囊开始营养限制，但这可能与较小的运动细胞产量，而不是一个特定的抑制包囊。相比之下，encystment是更敏感的温度比生长速度：最佳的包囊生产发生在一个相对较窄的温度范围内，没有包囊形成在某些温度下，允许增长。在培养基制备过程中引入的化学污染物也会降低孢囊产量。没有证据表明密度依赖性诱导机制，也没有在营养丰富的培养基中性行为和生长速率降低之间的联系。
Encystment of the toxic dinoflagellate G. tamarensis Lebour (var. excavata) was monitored in batch cultures exposed to a variety of nutritional and environmental treatments. Limitation by N (as ammonium or nitrate) or P (as phosphate) resulted in cyst formation. When the initial concentration of limiting nutrient was varied, total cyst yield (ml-1) was directly proportional to the cell yield at all but the highest nutrient concentrations (where encystment was minimal). Encystment efficiency was relatively constant (0.1-0.2 cysts/cell) over a 5-fold range of cell densities, indicating that 20-40% of the vegetative populations successfully encysted. Cyst formation was negligible in nutrient-replete medium, even with a significant reduction in growth rate due to non-optimal light, temperature, or to high batch culture cell densities. Low light levels decreased cyst yield once encystment was initiated by nutrient limitation, but this was probably linked to smaller motile cell yield and not to a specific inhibition of encystment. In contrast, encystment was more sensitive to temperature than was growth rate: optimal cyst production occurred over a relatively narrow temperature range and no cysts were formed at some temperatures that permitted growth. Cyst yield could also be reduced by chemical contaminants introduced during culture medium preparation. There is no evidence for a density-dependent induction mechanism, nor is there a link between sexuality and reduced growth rate in nutrient-replete medium.