Cross-reactive immune responses against Mycobacterium bovis BCG in mice infected with non-tuberculous mycobacteria belonging to the MAIS-Group
Cross-reactive immune responses against Mycobacterium bovis BCG in mice infected with non-tuberculous mycobacteria belonging to the MAIS-Group
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DOI：
10.1046/j.1365-3083.1997.d01-99.x
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发表时间：
1997-07-01
影响因子：
3.7
通讯作者：
Huygen, K
中科院分区：
文献类型：
--
作者：
Lozes, E;Denis, O;Huygen, K
用卡介苗敏感小鼠BALB/c和C57BL/6静脉感染细胞内分枝杆菌、绵羊分枝杆菌和鞭毛分枝杆菌，连续3个月观察卡介苗胞浆和分泌型抗原对分枝杆菌复制、抗体和Th1型细胞因子产生的影响。细胞内分枝杆菌和禽类分枝杆菌的初始集落形成单位(CFU)计数在肺中高于脾，而在囊状分枝杆菌中则相反。细胞内分枝杆菌是毒力最强的物种，其复制在两个小鼠品系中都无法控制。它还能诱导最强的抗体反应。在两个品系的小鼠体内，鸟分支杆菌均被消除，而在C57BL/6小鼠体内，球囊分枝杆菌最终被消除，但在BALB/c小鼠的脾中增殖。白介素2和干扰素-γ对卡介苗抗原的持续产生仅在微囊藻感染中被发现。与卡介苗接种一样，感染卡介苗的C57BL/6小鼠对整个卡介苗培养滤液、卡介苗提取物和纯化的BCG抗原85复合体(Ag85)的反应性高于BALB/c小鼠。这些数据表明，环境中斯氏分支杆菌的存在可能会干扰卡介苗遗传易感对象及其对结核分枝杆菌的保护作用。
Two bacillus Calmette-Guerin (BCG)-susceptible mouse strains, BALB/c and C57BL/6, were infected intravenously with Mycobacterium intracellulare, M. avium or M. scrofulaceum and monitored during 3 months for mycobacterial replication and antibody and Th1-type cytokine production in response to cytoplasmic and secreted antigens from M. bovis BCG. Whereas initial colony-forming unit (CFU) counts of M. intracellulare and M. avium were higher in lungs than in spleen, the opposite was observed for M. scrofulaceum. Mycobacterium intracellulare was the most virulent species and its replication could not be controlled in either mouse strain. It also induced the strongest antibody response. Mycobacterium avium was eliminated in both mouse strains and M. scofulaceum finally was eliminated in C57BL/6 but multiplied in spleen from BALB/c mice. Significant sustained interleukin-2 and interferon-gamma production towards BCG antigens was only found in M. scrofulaceum infection. As in BCG-vaccination, M. scrofulaceum-infected C57BL/6 mice demonstrated a higher response towards whole BCG culture filtrate, BCG extract and purified antigen 85 complex (Ag85) from BCG than did BALB/c mice. The data suggest that the presence of M. scrifulaceum in the environment may possibly interfere in genetically predisposed subjects with BCG vaccine and its protective efficacy against M. tuberculosis.