CD56 expression in feline lymphoid cells.

CD56 expression in feline lymphoid cells.
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猫淋巴细胞中 CD56 的表达。

DOI:
10.1292/jvms.65.769
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发表时间:
2003
期刊:
The Journal of veterinary medical science
影响因子:
--
通讯作者:
H. Akashi
H. Akashi
中科院分区:
--
文献类型:
--
作者:
M. Shimojima;Y. Nishimura;T. Miyazawa;Kentaro Kato;Y. Tohya;H. Akashi

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The neural-cell adhesion molecule (N-CAM) consists of three major types of polypeptides (180, 140, and 120 kDa) whose predominant differences exist within the transmembrane and cytoplasmic domains. In this study, we generated a monoclonal antibody, termed SZK1, reactive to feline CD56 (fCD56) molecules (140 kDa form of N-CAM) expressed by the baculovirus expression system and investigated fCD56 expression in feline lymphoid cells. In flow cytometric analysis, SZK1 was reactive to a feline T-lymphoblastoid cell line MYA-1. Further, SZK1 was reactive to a very small population (1.1-1.7%) of freshly isolated peripheral blood lymphocytes (PBLs) of three specific pathogen-free cats, and the reactivity was increased by culturing of PBLs in the presence of interleukin-2 following concanavalin A-stimulation (>10%). In immunoblotting analysis, SZK1 detected an approximately 160 kDa antigen from MYA-1 cells, while from RNA of the cells reverse transcription-polymerase chain reaction amplified the fragment resembling 140 kDa form of N-CAM. These finding suggest that fCD56 has similar characteristics with human CD56.
DOI: 10.4049/jimmunol.138.7.2019
发表时间: 1987-04
影响因子: 4.4
作者:
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发表时间: 1999
期刊: European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics
影响因子: --
作者:
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影响因子: 2.2
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