CD56 expression in feline lymphoid cells.
CD56 expression in feline lymphoid cells.
复制标题
猫淋巴细胞中 CD56 的表达。
DOI:
10.1292/jvms.65.769
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发表时间:
2003
期刊:
影响因子:
--
通讯作者:
H. Akashi
中科院分区:
文献类型:
--
作者:
M. Shimojima;Y. Nishimura;T. Miyazawa;Kentaro Kato;Y. Tohya;H. Akashi
The neural-cell adhesion molecule (N-CAM) consists of three major types of polypeptides (180, 140, and 120 kDa) whose predominant differences exist within the transmembrane and cytoplasmic domains. In this study, we generated a monoclonal antibody, termed SZK1, reactive to feline CD56 (fCD56) molecules (140 kDa form of N-CAM) expressed by the baculovirus expression system and investigated fCD56 expression in feline lymphoid cells. In flow cytometric analysis, SZK1 was reactive to a feline T-lymphoblastoid cell line MYA-1. Further, SZK1 was reactive to a very small population (1.1-1.7%) of freshly isolated peripheral blood lymphocytes (PBLs) of three specific pathogen-free cats, and the reactivity was increased by culturing of PBLs in the presence of interleukin-2 following concanavalin A-stimulation (>10%). In immunoblotting analysis, SZK1 detected an approximately 160 kDa antigen from MYA-1 cells, while from RNA of the cells reverse transcription-polymerase chain reaction amplified the fragment resembling 140 kDa form of N-CAM. These finding suggest that fCD56 has similar characteristics with human CD56.
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影响因子:
4.4
作者:
L. Lanier;A. Le;A. Ding;E. Evans;A. Krensky;C. Clayberger;J. Phillips
通讯作者:
L. Lanier;A. Le;A. Ding;E. Evans;A. Krensky;C. Clayberger;J. Phillips
影响因子:
56.9
作者:
CUNNINGHAM, BA;HEMPERLY, JJ;EDELMAN, GM
通讯作者:
EDELMAN, GM
DOI:
10.1046/j.1365-2370.1999.00121.x
发表时间:
1999
期刊:
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics
影响因子:
--
作者:
Nishimura,Y;Miyazawa,T;Ikeda,Y;Izumiya,Y;Nakamura,K;Cai,JS;Sato,E;Kohmoto,M;Mikami,T
通讯作者:
Mikami,T
DOI:
10.1073/pnas.83.24.9822
发表时间:
1986-12-01
影响因子:
11.1
作者:
HEMPERLY, JJ;EDELMAN, GM;CUNNINGHAM, BA
通讯作者:
CUNNINGHAM, BA
影响因子:
2.2
作者:
Hanlon,MA;Marr,JM;Hayes,KA;Mathes,LE;Stromberg,PC;Ringler,S;Krakowka,S;Lafrado,LJ
通讯作者:
Lafrado,LJ