Nitrogen starvation induces the accumulation of arachidonic acid in the freshwater green alga Parietochloris incisa (Trebuxiophyceae)
Nitrogen starvation induces the accumulation of arachidonic acid in the freshwater green alga Parietochloris incisa (Trebuxiophyceae)
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DOI：
10.1046/j.1529-8817.2002.01160.x
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发表时间：
2002-10-01
影响因子：
2.9
通讯作者：
Cohen, Z
中科院分区：
文献类型：
--
作者：
Khozin-Goldberg, I;Bigogno, C;Cohen, Z
绿藻顶绿藻尖梳状。nov （Trebuxiophyceae）是具有药用价值的长链多不饱和脂肪酸（PUFA）花生四烯酸最丰富的植物来源（20:46，AA）。超过90%的AA沉积在三酰基甘油（TAG）中。氮饥饿条件下，脂肪酸含量占干重的35%以上，AA含量占总脂肪酸的60%以上。因此，我们得到了超过20%的AA含量。据我们所知，这是藻类中PUFA含量最高的报告。随着生物量浓度的增加，AA比例和脂肪酸含量均有所提高。我们推测TAG在p.c incisa中的作用之一是作为可用于构建膜脂的AA的储存库。
The Chlorophyte Parietochloris incisa comb. nov (Trebuxiophyceae) was found to be the richest plant source of the pharmaceutically valuable long-chain polyunsaturated fatty acid (PUFA), arachidonic acid (20: 46, AA). Over 90% of total AA are deposited in triacylglycerols (TAG). Under nitrogen starvation, the fatty acid content constituted over 35% of dry weight and the proportion of AA exceeded 60% of total fatty acids. Consequently, we obtained an AA content of over 20%. This is, to the best of our knowledge, the highest reported content of any PUFA in algae. Increasing the biomass concentration resulted in an enhancement of both the proportion of AA and the fatty acid content. We hypothesize that one of the roles of TAG in P. incisa is to serve as a reservoir of AA that can be used for the construction of membranal lipids.