Detection and diversity of a putative novel heterogeneous polymorphic proline-glycine repeat (Pgr) protein in the footrot pathogen Dichelobacter nodosus
Detection and diversity of a putative novel heterogeneous polymorphic proline-glycine repeat (Pgr) protein in the footrot pathogen Dichelobacter nodosus
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DOI：
10.1016/j.vetmic.2010.06.024
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发表时间：
2011-01-27
影响因子：
3.3
通讯作者：
Wellington, Elizabeth M. H.
中科院分区：
文献类型：
--
作者：
Calvo-Bado, Leo A.;Green, Laura E.;Wellington, Elizabeth M. H.
结节双歧杆菌是一种革兰氏阴性厌氧菌，是引起绵羊蹄疾的重要病原。目前，根据该领域的临床表现，Footrot被描述为良性或毒力；结节螺旋体菌株也被归类为良性或毒力，但这种命名并不总是与临床疾病一致。本研究的目的是确定编码一个可能的脯氨酸-甘氨酸重复蛋白(PGR)的PGR基因的多样性。PgR基因存在于所有100个被检测的节瘤乳杆菌分离株中，根据序列分析，它有两个变异体，即pgrA和pgrB。在pgrA中，有两个编码串联重复序列的区域，即R1和R2：不同的菌株在这些区域内具有不同数量的重复序列。PgrB中没有R1和R2。这两个变种在澳大利亚、瑞典和英国的菌株中都存在，然而，在西澳大利亚的分离株中只检测到pgrB。在英国两个有致命脚步的鸡群的组织样本中发现了pgrA基因，而在10年内没有剧毒或良性脚步的鸡群中只检测到了pgrB基因。对推测的PgrA蛋白的生物信息学分析表明，它含有一个胶原样细胞表面锚定基序。这些结果表明，PGR基因可能是流行病学研究中有用的分子标记。(C)2010爱思唯尔B.V.保留所有权利。
Dichelobacter nodosus, a Gram-negative anaerobic bacterium, is the essential causative agent of footrot in sheep. Currently, depending on the clinical presentation in the field, footrot is described as benign or virulent; D. nodosus strains have also been classified as benign or virulent, but this designation is not always consistent with clinical disease. The aim of this study was to determine the diversity of the pgr gene, which encodes a putative proline-glycine repeat protein (Pgr). The pgr gene was present in all 100 isolates of D. nodosus that were examined and, based on sequence analysis had two variants, pgrA and pgrB. In pgrA, there were two coding tandem repeat regions, R1 and R2: different strains had variable numbers of repeats within these regions. The R1 and R2 were absent from pgrB. Both variants were present in strains from Australia, Sweden and the UK, however, only pgrB was detected in isolates from Western Australia. The pgrA gene was detected in D. nodosus from tissue samples from two flocks in the UK with virulent footrot and only pgrB from a flock with no virulent or benign footrot for >10 years. Bioinformatic analysis of the putative PgrA protein indicated that it contained a collagen-like cell surface anchor motif. These results suggest that the pgr gene may be a useful molecular marker for epidemiological studies. (C) 2010 Elsevier B.V. All rights reserved.