Amphiregulin Induces Proliferative Activities in Osseous Dysplasia
Amphiregulin Induces Proliferative Activities in Osseous Dysplasia
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DOI：
10.1177/0022034509338253
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发表时间：
2009-06-01
影响因子：
7.6
通讯作者：
Kamata, N.
中科院分区：
文献类型：
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作者：
Shigeishi, H.;Yamaguchi, S.;Kamata, N.
人类骨发育异常（OD）是一种源于牙周膜的良性纤维骨性肿瘤，在该病症中，正常骨质被含有异常骨或牙骨质的纤维结缔组织所替代。然而，OD中的细胞分化与增殖机制尚未完全阐明。体外培养系统在分析研究方面具有显著优势。因此，我们从一名患有颌骨干发育异常（GDD）个体的颌骨OD病变组织中建立了永生化细胞系（OD - 1）。我们假设OD - 1具有与成骨细胞等矿化相关细胞不同的特征性生长机制。为了阐明OD - 1与成骨细胞之间基因表达模式的差异，我们通过微阵列分析比较了这两种细胞类型中表达的基因谱。我们发现，与成骨细胞和牙龈成纤维细胞相比，双调蛋白在OD - 1中高表达。OD - 1表现出由双调蛋白以自分泌方式调节的增殖活性，并且双调蛋白可能在OD的增殖过程中发挥重要作用。
Human osseous dysplasia (OD) is a benign fibroosseous neoplasm of periodontal ligament origin in which normal bone is replaced with fibrous connective tissue containing abnormal bone or cementum. However, cellular differentiation and proliferation in OD have not been fully elucidated. In vitro culture systems have distinct advantages for analytical studies. Therefore, we established immortalized cell lines (OD-1) from OD lesions of the jaw from an individual with gnatho-diaphyseal dysplasia (GDD). We hypothesized that OD-1 had a characteristic growth mechanism different from that of mineralized-associated cells such as osteoblasts. To clarify the difference of gene expression patterns between OD-1 and osteoblasts, we compared the profiles of genes expressed in the 2 cell types by microarray analysis. We identified amphiregulin to be highly expressed in OD-1 compared with osteoblasts and gingival fibroblasts. OD-1 showed proliferative activities regulated in an autocrine manner by amphiregulin, and amphiregulin may play a significant role in the proliferation of OD.