Alveolar bone regeneration of subcutaneously transplanted rat molar
Alveolar bone regeneration of subcutaneously transplanted rat molar
复制标题
DOI：
10.1016/j.bone.2007.09.054
复制
发表时间：
2008-02-01
期刊：
影响因子：
4.1
通讯作者：
Nakamura, Hiroaki
中科院分区：
文献类型：
--
作者：
Hosoya, Akihiro;Ninomiya, Tadashi;Nakamura, Hiroaki
牙槽骨再生对牙周治疗至关重要。近来，细胞替代疗法已被关注于牙周病，但再生牙槽骨的细胞来源仍不确定。因此，为了弄清这些骨再生细胞的来源，我们将转基因gfp大鼠磨牙移植到野生型大鼠皮下组织中。移植5天后，牙齿被含有许多血管的结缔组织包围。10 d时，分岔根分支间的结缔组织形成骨样组织。20天后，硬组织扩展到几乎所有分叉区，无骨性强直。在新形成的基质中，所有成骨细胞样细胞对GFP均呈免疫阳性。此外，这些细胞和基质外周细胞对BMP4、Runx2、BSP和OPN表现出强烈的免疫反应性。这些结果表明，牙周韧带组织含有骨祖细胞，具有再生牙槽骨的能力。我们的模型表明，这些再生过程可能类似于正常的牙槽骨形成。(c) 2007爱思唯尔公司版权所有。
Regeneration of alveolar bone is essential for periodontal treatment. Recently, cell replacement therapy has been focused on periodontal disease, but the source of the cells that regenerate alveolar bone is still uncertain. Therefore, to clarify the source of these bone-regenerating cells, we transplanted GFP-transgenic rat molars into the subcutaneous tissues of wild-type rats. Five days after transplantation, the tooth was surrounded by connective tissue containing many blood vessels. At 10 days, bone-like tissue was formed in the connective tissue between the branches of the bifurcated root. This hard tissue expanded to almost all of this bifurcation area without osseous ankylosis after 20 days. All ostcoblast-like cells in the newly formed matrix were immunopositive for GFP. In addition, these cells and the peripheral cells of the matrix showed intense immunoreactivity for BMP4, Runx2, BSP, and OPN. These results demonstrate that periodontal ligament tissue contains osteoprogenitor cells that have the ability to regenerate alveolar bone. Our model suggests that these regeneration processes might be similar to normal alveolar bone formation. (c) 2007 Elsevier Inc. All rights reserved.