Overexpression of an Orchid (Dendrobium nobile) SOC1/TM3-Like Ortholog, DnAGL19, in Arabidopsis Regulates HOS1-FT Expression.
Overexpression of an Orchid (Dendrobium nobile) SOC1/TM3-Like Ortholog, DnAGL19, in Arabidopsis Regulates HOS1-FT Expression.
复制标题
DOI：
10.3389/fpls.2016.00099
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Liang S
中科院分区：
文献类型：
--
作者：
Liu XR;Pan T;Liang WQ;Gao L;Wang XJ;Li HQ;Liang S
在适当的季节开花是被子植物成功繁殖的关键。兰花品种，金钗石斛，需要春化，以实现在春天开花，但基本的监管网络尚未确定的日期。MADS盒转录因子DnAGL 19先前在低温处理的D.并建议调节春化诱导开花。在这项研究中，系统发育分析的DnAGL 9和MADS-box包含的蛋白质表明，DnAGL 19是在遗传学上密切相关的SOC 1-like蛋白从兰花石斛Chao Parya微笑，DOSOC 1。兰花分支接近但不包括在与真双子叶植物或单子叶植物相关的SOC 1 -1/TM 3分支中，表明DnAGL 19是SOC 1 -1/TM 3-like直系同源物。DnAGL 19被认为是高表达的假鳞茎，叶，根，腋芽，但很少在花，并大幅上调腋芽长时间的低温处理。DnAGL 19在拟南芥中的过表达导致了一个小的，但显着减少的时间抽薹，表明开花时间略有加快在正常生长条件下。与此相一致，A. APETELA 1（AP 1）基因在转基因植株中的表达比野生型植株早，而FLOWLOCUS T（FT）基因被抑制，表明这些转录因子的调控改变导致了对开花的弱促进作用。在相同条件下，渗透反应基因1（HOS 1）的高表达被轻微激活，表明HOS 1-FT模块可能参与DnAGL 19相关网络。在春化条件下，FT表达显著上调，而转基因A. thaliana具有与野生型相似的水平。综上所述，这些结果表明DnAGL 19根据温度线索控制HOS 1-FT模块的作用，这可能有助于D.高贵的开花时间这些数据提供了深入了解开花是如何微调D。使植物适应季节性的温度变化。
Flowering in the appropriate season is critical for successful reproduction in angiosperms. The orchid species, Dendrobium nobile, requires vernalization to achieve flowering in the spring, but the underlying regulatory network has not been identified to date. The MADS-box transcription factor DnAGL19 was previously identified in a study of low-temperature treated D. nobile buds and was suggested to regulate vernalization-induced flowering. In this study, phylogenetic analysis of DnAGL9 and the MADS-box containing proteins showed that DnAGL19 is phylogenetically closely related to the SOC1-like protein from orchid Dendrobium Chao Parya Smile, DOSOC1. The orchid clade closed to but is not included into the SOC1-1/TM3 clades associated with either eudicots or monocots, suggesting that DnAGL19 is an SOC1-1/TM3-like ortholog. DnAGL19 was found to be highly expressed in pseudobulbs, leaves, roots, and axillary buds but rarely in flowers, and to be substantially upregulated in axillary buds by prolonged low-temperature treatments. Overexpression of DnAGL19 in Arabidopsis thaliana resulted in a small but significantly reduced time to bolting, suggesting that flowering time was slightly accelerated under normal growth conditions. Consistent with this, the A. thaliana APETELA1 (AP1) gene was expressed at an earlier stage in transgenic lines than in wild type plants, while the FLOWERING LOCUS T (FT) gene was suppressed, suggesting that altered regulations on these transcription factors caused the weak promotion of flowering. HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 (HOS1) was slightly activated under the same conditions, suggesting that the HOS1-FT module may be involved in the DnAGL19-related network. Under vernalization conditions, FT expression was significantly upregulated, whereas HOS1 expression in the transgenic A. thaliana has a level similar to that in wild type. Taken together, these results suggest that DnAGL19 controls the action of the HOS1-FT module depending on temperature cues, which could contribute to regulation of D. nobile flowering time. These data provide insights into how flowering is fine-tuned in D. nobile to acclimate the plant to seasonal changes in temperature.