Effect of dietary omega-3 fatty acids and chronic ethanol consumption on reverse cholesterol transport in rats
Effect of dietary omega-3 fatty acids and chronic ethanol consumption on reverse cholesterol transport in rats
复制标题
DOI：
10.1016/s0026-0495(00)80017-7
复制
发表时间：
2000-04-01
影响因子：
9.8
通讯作者：
Lakshman, MR
中科院分区：
文献类型：
--
作者：
Marmillot, P;Rao, MN;Lakshman, MR
我们之前的研究表明，慢性乙醇喂养会降低高密度脂蛋白（HDL）中的载脂蛋白E (apoE)，而在这种饮食中补充总膳食热量的2.8%作为omega - 3脂肪酸（omega 3FAs），可使HDL-apoE恢复到控制值。由于含有载脂蛋白e的高密度脂蛋白在逆向胆固醇转运（RCT）中起着重要作用，我们在本研究中测量了慢性乙醇摄入和omega 3-FAs -FAs对RCT的影响。对照组正常脂肪（CN）、酒精-正常脂肪（AN）、对照组欧米伽- 3FA脂肪（CF）和酒精-欧米伽- 3FA脂肪（AF）四组大鼠分别饲喂各自的饲料8周，之后评估各组肝细胞和hdl的RCT容量（肝细胞摄取巨噬细胞排出的胆固醇）。与对照饲粮（CN）相比，慢性乙醇（AN）喂养使HDL的胆固醇外排能力降低了21% (P
We previously showed that chronic ethanol feeding reads to a decrease of apolipoprotein E (apoE) in high-density lipoprotein (HDL), whereas supplementing this diet with 2.8% of total dietary calories as omega 3-fatty acids (omega 3FAs) restores HDL-apoE to the control values. Since HDL containing apoE plays a major role in reverse cholesterol transport (RCT), we measured the effects chronic ethanol intake and omega 3-FAs -FAs on RCT in the present study. Four groups of rats, control normal fat (CN), alcohol-normal fat (AN), control omega 3FA fat (CF), and alcohol-omega 3FA fat (AF), were fed their respective diets for 8 weeks, after which hepatocytes and HDLs from each group were evaluated for RCT capacity (cholesterol efflux from macrophages end uptake by liver cells). Compared with the control diet (CN), chronic ethanol (AN) feeding inhibited the cholesterol efflux capacity of HDL by 21% (P