C3 and 2D C3 Marfey's Methods for Amino Acid Analysis in Natural Products
C3 and 2D C3 Marfey's Methods for Amino Acid Analysis in Natural Products
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DOI：
10.1021/acs.jnatprod.5b01125
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发表时间：
2016-02-01
影响因子：
5.1
通讯作者：
Capon, Robert J.
中科院分区：
文献类型：
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作者：
Vijayasarathy, Soumini;Prasad, Pritesh;Capon, Robert J.
我们验证了 C-3 Marfey 方法改进的分辨率和灵敏度，包括针对天然产物中常见的一系列氨基酸以及与现有 Marfey 方法进行比较来解析所有 Ile 异构体的能力。我们还描述了一种创新的 2D C-3 Marfey 方法，作为确定天然产物中对映体氨基酸残基区域化学的分析方法。 C-3 和 2D C-3 Marfey 方法代表了探索和定义天然产物中可水解氨基酸残基的立体复杂性的宝贵工具。
We validate the improved resolution and sensitivity of the C-3 Marfey's method, including an ability to resolve all Ile-isomers, against an array of amino acids commonly encountered in natural products and by comparison to an existing Marfey's method. We also describe an innovative 2D C-3 Marfey's method as an analytical approach for determining the regiochemistry of enantiomeric amino acid residues in natural products. The C-3 and 2D C-3 Marfey's methods represent valuable tools for probing and defining the stereocomplexity of hydrolytically accessible amino acid residues in natural products.