The effect of extracts from ginger rhizome on inflammatory mediator production
The effect of extracts from ginger rhizome on inflammatory mediator production
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DOI：
10.1016/j.phymed.2006.03.003
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发表时间：
2007-01-01
期刊：
影响因子：
7.9
通讯作者：
Timmermann, B. N.
中科院分区：
文献类型：
--
作者：
Lantz, R. C.;Chen, G. J.;Timmermann, B. N.
来自通常被称为姜的药用植物的根茎的化合物据称具有抗炎作用。我们已经使用体外测试系统来测试从生姜根茎中分离的化合物的抗炎活性。使U937细胞分化，并在存在或不存在有机提取物或在姜中发现的标准化合物（6-、8-、10-姜辣素或6-姜烯酚）的情况下暴露于来自大肠杆菌的脂多糖（LPS）（1 μ g/ml）24小时。收集上清液并通过标准ELISA测定分析前列腺素E-2（PGE（2））和肿瘤坏死因子α（TNF-α）的产生。有机提取物中的主要化合物被鉴定为6-，8- 10-姜辣素和6-，8-，10-姜烯酚。含有姜辣素的有机提取物或标准品不具有细胞毒性，而主要含有姜烯酚的提取物或标准品在浓度高于20 μ g/ml时具有细胞毒性。生姜有机粗提物能抑制LPS诱导的PGE（2）的产生（IC_（50）< 0.1 μ g/ml）。然而，提取物在抑制TNF-α（IC 50> 30 μ g/ml）方面几乎没有那么有效。通过柱色谱法制备了33个组分和亚组分，分析了其生物活性。主要含有姜辣素或姜烯酚的提取物（通过HPLC鉴定）在抑制LPS诱导的PGE（2）产生方面都具有高度活性（IC 50 < 0.1 μ g/ml），而含有未知化合物的提取物效果较差（IC 50 < 3.2 μ g/ml）。主要含有姜辣素的提取物或标准品能够抑制LPS诱导的考克斯-2表达，而含有姜烯酚的提取物对考克斯-2表达没有影响。这些数据表明，生姜中发现的化合物能够抑制PGE（2）的产生，并且这些化合物可能在几个部位起作用。(c)2006年Elsevier GmbH。All rights reserved.
Compounds from rhizomes of Zingiber officinale, commonly called ginger, have been purported to have anti-inflammatory actions. We have used an in vitro test system to test the anti-inflammatory activity of compounds isolated from ginger rhizome. U937 cells were differentiated and exposed to lipopolysaccharide (LPS) from Escherichia coli (I mu g/ml) in the presence or absence of organic extracts or standard compounds found in ginger (6-, 8-, 10-gingerol or 6-shogaol) for 24 h. Supernatants were collected and analyzed for the production of prostaglandin E-2, (PGE(2)) and tumor necrosis factor alpha (TNF-alpha) by standard ELISA assays. Predominant compounds in the organic extracts were identified as 6-, 8- 10-gingerols and 6-, 8-, 10-shogaols. Organic extracts or standards containing gingerols were not cytotoxic, while extracts or standards containing predominantly shogaols were cytotoxic at concentrations above 20 mu g/ml. Crude organic extracts of ginger were capable of inhibiting LPS induced PGE(2) (IC50 < 0.1 mu g/ml) production. However, extracts were not nearly as effective at inhibiting TNF-alpha (IC50 > 30 mu g/ml). Thirty three fractions and subfractions, prepared by column chromatography, were analyzed for bioactivity. Extracts containing either predominantly gingerols or shogaols (identified by HPLC) were both highly active at inhibiting LPS-induced PGE(2) production (IC50 < 0.1 mu g/ml), while extracts that contained unknown compounds were less effective (IC50 < 3.2 mu g/ml). Extracts or standards containing predominantly gingerols were capable of inhibiting LPS-induced COX-2 expression while shogaol containing extracts had no effect on COX-2 expression. These data demonstrate that compounds found in ginger are capable of inhibiting PGE(2) production and that the compounds may act at several sites. (c) 2006 Elsevier GmbH. All rights reserved.