Differential Detection of A, B and H Antigens in Semen and Saliva Stains Detected by an Elisa Technique

Differential Detection of A, B and H Antigens in Semen and Saliva Stains Detected by an Elisa Technique
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Elisa 技术检测精液和唾液染色中 A、B 和 H 抗原的差异检测

DOI:
10.1007/978-3-642-75496-8_49
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发表时间:
1990
期刊:
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通讯作者:
K. D. Soyza
K. D. Soyza
中科院分区:
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文献类型:
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作者:
K. D. Soyza

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在我们的实验室中,我们修改了弗莱彻(1989)描述的酶联免疫吸附测定法,并验证了它在法医样品中的应用。作为验证工作的一部分,对A、B和H抗原的不同水平进行了研究,以设定一个检测其中一种抗原的阈值,在该阈值下,未检测到其他抗原可视为其真实缺失。这在对法医工作中经常遇到的弱染色剂进行分组时尤为重要。
In our laboratory we have modified an enzyme linked immunosorbent assay described by Fletcher (1989); and validated it for use in Forensic Samples. As part of the validation exercise a study was made of the differential levels of A, B and H antigens, in order to set a threshold value for the detection of one of the antigens at which the non detection of the others could be taken as their true absence. This is particularly important when grouping weak stains that are often encountered in Forensic work.