Role of the 14-3-3 protein in carbon metabolism of the pathogenic yeast Candida albicans.
Role of the 14-3-3 protein in carbon metabolism of the pathogenic yeast Candida albicans.
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14-3-3 蛋白在病原酵母白色念珠菌碳代谢中的作用。
DOI：
10.1002/yea.1079
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Rustchenko,Elena
中科院分区：
文献类型：
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作者：
Wang,Ying-Kai;Das,Biswadip;Huber,DavidH;Wellington,Melanie;Kabir,MAnaul;Sherman,Fred;Rustchenko,Elena
我们以前证明，致病性酵母菌白色念珠菌通过一种新的机制有效地适应利用L-山梨糖（Sou+），这种机制基于5号染色体的一个拷贝的丢失，可能是由于位于该染色体上的负调节因子的拷贝数减少。我们在这里报告了另一个L-山梨糖利用的负调节因子，酿酒酵母BMH 1基因的直向同源物，该基因编码进化上保守的蛋白质14-3-3。这个必需基因位于1号染色体上，没有旁系同源物，据推测是调控网络的一个组成部分。涉及破坏BMH 1的一个等位基因和过表达BMH 1的实验表明，BMH 1抑制SOU 1的转录，SOU 1负责L-山梨糖的利用。虽然BMH 1和SOU 1之间相互作用的确切机制尚不清楚，但很明显，BMH 1的控制是基于基因拷贝数的比例，BMH 1不控制5号染色体的丢失，这是产生Sou+突变体的主要机制。我们认为BMH 1作为SOU 1的负调节因子的功能有助于细胞内环境的稳定。这是第一次报告的作用，C。白念珠菌必需基因BMH 1是酵母利用次级碳源的负调控因子，进一步证实了14-3-3蛋白参与多种功能。版权所有© 2004年约翰威利父子有限公司。
We previously demonstrated that the pathogenic yeastCandida albicanseffectively adapts to utilizeL‐sorbose (Sou+) by a novel mechanism based on the loss of one copy of chromosome 5, probably due to the reduction of copy number of a negative regulator located on this chromosome. We report here another negative regulator ofL‐sorbose utilization, an orthologue of theSaccharomyces cerevisiae BMH1gene, which encodes the evolutionarily conserved protein 14–3–3. This essential gene is located on chromosome 1, does not have paralogues, and is supposedly a component of the regulatory network. Experiments involving disruption of one allele ofBMH1and overexpression ofBMH1revealed thatBMH1represses the transcription ofSOU1, which is responsible for the utilization ofL‐sorbose. Although the exact mechanism of the interaction betweenBMH1andSOU1is not known, it is clear that the control is based on the ratio of gene copy number, and thatBMH1does not control the loss of chromosome 5, the major mechanism producing Sou+mutants. We propose that function ofBMH1as a negative regulator ofSOU1contributes to a general cellular homeostasis. This is a first report on the role of theC. albicansessential geneBMH1as a negative regulator of the utilization of secondary carbon source in yeast, which further substantiates the involvement of 14–3–3 proteins in diverse functions. Copyright © 2004 John Wiley & Sons, Ltd.