MiR-17-5p Up-Regulates YES1 to Modulate the Cell Cycle Progression and Apoptosis in Ovarian Cancer Cell Lines (Retracted article. See vol. 122, 2021)
MiR-17-5p Up-Regulates YES1 to Modulate the Cell Cycle Progression and Apoptosis in Ovarian Cancer Cell Lines (Retracted article. See vol. 122, 2021)
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DOI：
10.1002/jcb.25060
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发表时间：
2015-06-01
影响因子：
4
通讯作者：
Tang, Hua
中科院分区：
文献类型：
--
作者：
Li, Lan;He, Li;Tang, Hua
MicroRNAs （miRNAs）是参与基因表达调控的小的非编码rna。尽管许多研究表明miR-17-5p参与不同的癌症，但其在卵巢癌中的功能知之甚少。在这项研究中，我们证明了在ES-2和OVCAR3细胞系中，过表达miR-17-5p能够通过促进细胞周期G1/S转变和抑制细胞凋亡来增强细胞增殖，而抑制miR-17-5p则产生相反的表型。YES1被鉴定为miR-17-5p的一个新的靶基因。此外，miR-17-5p被发现直接结合YES1 mRNA的30UTR并上调其表达。此外，YES1的敲低导致ES-2和OVCAR3细胞增殖抑制和诱导细胞周期阻滞。YES1的异位表达能够逆转miR-17-5p抑制的作用。综上所述，我们的研究结果表明miR-17-5p可能通过上调YES1的表达在人卵巢癌中发挥作用。(C) 2015 Wiley期刊公司
MicroRNAs (miRNAs) are small, non-coding RNAs that participate in the regulation of gene expression. Although many studies have demonstrated the involvement of miR-17-5p in different cancers, little is known to its function in ovarian cancer. In this study, we demonstrated that overexpression of miR-17-5p was able to enhance cell proliferation by promoting G1/S transition of the cell cycle and suppressing apoptosis in ES-2 and OVCAR3 cell lines, whereas inhibition of miR-17-5p yielded the reverse phenotype. YES1 was identified as a novel target gene of miR-17-5p. Moreover, miR-17-5p was found to directly bind to the 30UTR of YES1 mRNA and up-regulated its expression. Furthermore, knockdown of YES1 led to the suppression of proliferation and induced cell cycle arrest in ES-2 and OVCAR3 cells. Ectopic expression of YES1 was able to reverse the effects of miR-17-5p inhibition. Collectively, our results indicated that miR-17-5p might play a role in human ovarian cancer by up-regulating YES1 expression. (C) 2015 Wiley Periodicals, Inc.