Accurate quantification of lipid species by electrospray ionization mass spectrometry - Meet a key challenge in lipidomics.
Accurate quantification of lipid species by electrospray ionization mass spectrometry - Meet a key challenge in lipidomics.
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DOI：
10.3390/metabo1010021
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发表时间：
2011-12-01
期刊：
影响因子：
4.1
通讯作者：
Han X
中科院分区：
文献类型：
--
作者：
Yang K;Han X
电喷雾电离质谱 (ESI-MS) 已成为脂质组学中识别和量化单个脂质种类的最流行和最强大的技术之一。同时，ESI-MS对脂质种类的定量分析也成为应对脂质组学挑战的主要障碍。在此，我们讨论不同基于质谱的脂质定量方法的原理、优点和可能的局限性，以及对于准确定量单个脂质种类重要的一些实际问题。因此，脂质组学的关键挑战之一——单个脂质种类的准确定量实际上可以得到满足。
Electrospray ionization mass spectrometry (ESI-MS) has become one of the most popular and powerful technologies to identify and quantify individual lipid species in lipidomics. Meanwhile, quantitative analysis of lipid species by ESI-MS has also become a major obstacle to meet the challenges of lipidomics. Herein, we discuss the principles, advantages, and possible limitations of different mass spectrometry-based methodologies for lipid quantification, as well as a few practical issues important for accurate quantification of individual lipid species. Accordingly, accurate quantification of individual lipid species, one of the key challenges in lipidomics, can be practically met.