Escherichia coli sterilization and lipopolysaccharide inactivation using microwave-induced argon plasma at atmospheric pressure
Escherichia coli sterilization and lipopolysaccharide inactivation using microwave-induced argon plasma at atmospheric pressure
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DOI：
10.1016/j.surfcoat.2006.07.039
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发表时间：
2007-02
影响因子：
5.4
通讯作者：
B. Park;K. Takatori;Mi Hee Lee;Dongwook Han;Yeon I Woo;H. Son;J. Kim;Kie-Hyung Chung;Soon O. Hyun;Jong-Chul Park
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文献类型：
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作者：
B. Park;K. Takatori;Mi Hee Lee;Dongwook Han;Yeon I Woo;H. Son;J. Kim;Kie-Hyung Chung;Soon O. Hyun;Jong-Chul Park
在制造医疗器械和生物材料时，对人体有害的微生物的灭活是非常重要的。更重要的是从医疗器械中去除杀灭的细菌及其碎片，特别是热原，如脂多糖（LPS）。LPS，也称为内毒素，来源于革兰氏阴性细菌的细胞壁，并且众所周知可诱导各种病理性疾病。本研究对大肠杆菌（Escherichia coli，E. coli）和消除E.利用自行设计的微波诱导等离子体系统，在常压下对大肠杆菌来源的LPS进行了检测。等离子体处理能完全杀灭大肠杆菌。大肠杆菌在1s后，这是通过形态学改变，如破裂的膜和无定形结构证实。此外，LPS在等离子体处理10秒后完全失活。这些结果表明，微波诱导等离子体不仅对大肠杆菌有效。大肠杆菌灭菌，但也将能够从被灭菌物体的表面去除LPS。
Upon fabricating medical devices and biomaterials, the inactivation of microorganisms harmful to human is very important. It is more important to remove killed bacteria and their debris, particularly pyrogens such as lipopolysaccharide (LPS) from medical devices. LPS, referred to as endotoxin, is derived from the cell wall of gram-negative bacteria and well-known for inducing various pathological diseases. In this study, the sterilization of Escherichia coli (E. coli) and elimination of E. coli-derived LPS were examined by using our self-designed microwave-induced plasma system at atmospheric pressure. Plasma treatment resulted in complete sterilization of E. coli after 1 s, which was confirmed by morphological alterations such as ruptured membranes and amorphous structures. Furthermore, LPS was completely inactivated after 10 s of plasma treatment. These results suggest that microwave-induced plasma will not only be effective on E. coli sterilization, but will also be able to remove LPS from the surface of the objects being sterilized.