Genetically engineered zebrafish as models of skeletal development and regeneration
Genetically engineered zebrafish as models of skeletal development and regeneration
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DOI：
10.1016/j.bone.2022.116611
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发表时间：
2022-12-05
期刊：
影响因子：
4.1
通讯作者：
Youngstrom，Daniel W.
中科院分区：
文献类型：
--
作者：
Henke，Katrin;Farmer，D'Juan T.;Youngstrom，Daniel W.
斑马鱼（Danio rerio）是一种水生脊椎动物，与陆生动物具有显著的同源性。虽然斑马鱼在发育和再生生物学方面有着长达几个世纪的记录，但随着现代遗传学的出现，它们的用途呈指数级增长。这在专注于骨骼发育和修复的研究中得到了例证。在此，斑马鱼的许多贡献，我们的理解软骨，骨，肌腱/韧带，和其他骨骼组织的基础科学进行了描述，特别关注的应用程序的发展和再生。我们总结了遗传优势，使斑马鱼的一个强大的模型，以了解骨骼生物学。我们还强调了现有的大量工具和技术可用于了解斑马鱼的骨骼发育和修复，并介绍了新兴的方法，这将有助于骨骼生物学的新发现。最后，我们回顾了斑马鱼对我们理解再生的独特贡献，并强调了在不同损伤情况下的不同修复途径。我们的结论是，斑马鱼将继续填补一个利基不断增加的广度和深度研究的基本细胞机制的骨骼生物学。
Zebrafish (Danio rerio) are aquatic vertebrates with significant homology to their terrestrial counterparts. While zebrafish have a centuries-long track record in developmental and regenerative biology, their utility has grown exponentially with the onset of modern genetics. This is exemplified in studies focused on skeletal development and repair. Herein, the numerous contributions of zebrafish to our understanding of the basic science of cartilage, bone, tendon/ligament, and other skeletal tissues are described, with a particular focus on applications to development and regeneration. We summarize the genetic strengths that have made the zebrafish a powerful model to understand skeletal biology. We also highlight the large body of existing tools and techniques available to understand skeletal development and repair in the zebrafish and introduce emerging methods that will aid in novel discoveries in skeletal biology. Finally, we review the unique contributions of zebrafish to our understanding of regeneration and highlight diverse routes of repair in different contexts of injury. We conclude that zebrafish will continue to fill a niche of increasing breadth and depth in the study of basic cellular mechanisms of skeletal biology.