GLYCOLYSIS-INDUCED DISCORDANCE BETWEEN GLUCOSE METABOLIC RATES MEASURED WITH RADIOLABELED FLUORODEOXYGLUCOSE AND GLUCOSE
GLYCOLYSIS-INDUCED DISCORDANCE BETWEEN GLUCOSE METABOLIC RATES MEASURED WITH RADIOLABELED FLUORODEOXYGLUCOSE AND GLUCOSE
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DOI：
10.1038/jcbfm.1989.111
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发表时间：
1989-12-01
影响因子：
6.3
通讯作者：
LEAR, JL
中科院分区：
文献类型：
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作者：
ACKERMANN, RF;LEAR, JL
我们已经开发了一种放射自显影方法，用于估计局部CMRglc（LCMRglc）的氧化和糖酵解组分，使用顺序给药的[18 F]氟脱氧葡萄糖（FDG）和[14 C]-6-葡萄糖（GLC）。FDG-6-磷酸积累与葡萄糖磷酸化的速率成比例，葡萄糖磷酸化发生在糖酵解（GMg）和氧化（GMo）葡萄糖代谢的分歧之前，因此与总脑葡萄糖代谢GMt相关：GMg + GMo = GMt。 在氧化代谢的情况下，GLC的14 C标记暂时保留在克雷布斯循环相关底物库中。我们假设，糖酵解代谢，然而，一个显着的14 C标签的一部分是从大脑中丢失通过乳酸的生产和流出大脑。因此，脑GLC代谢物浓度可能与GMo的关系比与GMt的关系更密切。如果为真，则糖酵解代谢率将与FDG和GLC衍生的LCMRglc之间的差异相关。到目前为止，我们已经研究了正常清醒的大鼠，与海人酸（KA）诱导的边缘激活，和大鼠视觉刺激16赫兹闪光。在KA治疗的大鼠，FDG和GLC积累，我们归因于糖酵解之间的显着不一致，只发生在激活的边缘结构。在视觉刺激的大鼠，显着的不一致只发生在视顶盖。
We have developed an autoradiographic method for estimating the oxidative and glycolytic components of local CMRglc (LCMRglc), using sequentially administered [18F]fluorodeoxyglucose (FDG) and [14C]-6-glucose (GLC). FDG-6-phosphate accumulation is proportional to the rate of glucose phosphorylation, which occurs before the divergence of glycolytic (GMg) and oxidative (GMo) glucose metabolism and is therefore related to total cerebral glucose metabolism GMt: GMg + GMo = GMt. With oxidative metabolism, the 14C label of GLC is temporarily retained in Krebs cycle-related substrate pools. We hypothesize that with glycolytic metabolism, however, a significant fraction of the 14C label is lost from the brain via lactate production and efflux from the brain. Thus, cerebral GLC metabolite concentration may be more closely related to GMo than to GMt. If true, the glycolytic metabolic rate will be related to the difference between FDG- and GLC-derived LCMRglc. Thus far, we have studied normal awake rats, with limbic activation induced by kainic acid (KA), and rats visually stimulated with 16-Hz flashes. In KA-treated rats, significant discordance between FDG and GLC accumulation which we attribute to glycolysis, occurred only in activated limbic structures. In visually stimulated rats, significant discordance occurred only in the optic tectum.