A highly sensitive enzyme catalytic SERS quantitative analysis method for ethanol with Victoria blue B molecular probe in the stable nanosilver sol substrate
A highly sensitive enzyme catalytic SERS quantitative analysis method for ethanol with Victoria blue B molecular probe in the stable nanosilver sol substrate
复制标题
稳定纳米银溶胶基质中维多利亚蓝B分子探针高灵敏酶催化SERS定量分析乙醇方法
DOI：
10.1016/j.snb.2017.09.177
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Jiang Zhiliang
中科院分区：
文献类型：
--
作者：
Li Chongning;Qin Yanna;Li Dan;Zhang Chi;Liang Aihui;Wen Guiqing;Lu Zujun;Jiang Zhiliang
以AgNO 3为前驱体，采用光波法快速制备了稳定的银纳米溶胶，并对其表面增强拉曼光谱（Sers）活性进行了研究。将乙醇氧化酶催化反应与Fe 2+催化反应耦合，产生的羟基自由基不仅氧化纳米银表面的银原子，形成分散度更高、粒径更小、Sers效应较弱的纳米银，而且氧化分子探针维托里亚蓝B（VBB）褪色，建立了一种高灵敏度的Sers定量分析方法。随着乙醇浓度的增加，纳米银和探针分子被氧化的数量越多，导致1615 cm-1处的Sers强度下降。乙醇浓度在0.008-0.40 mg/L范围内与Sers强度呈良好的线性关系，检测限为3 μg/L。方法用于啤酒和血清中乙醇的测定，结果满意.
Using AgNO3as precursor,a stable silver nanosol with strong SERS activity was prepared rapidly by light-wave procedure. A highly sensitive SERS quantitative analysis method for ethanol was developed, coupled the alcohol oxidase catalytic reaction with the Fe2+catalytic reaction that the produced hydroxyl radicals not only oxidized the silver atoms on the nanosilver surface to form more dispersed and small size nanosilvers with weak SERS effect, but also oxidized the molecular probe Vitoria blue B (VBB) to fade. With the increasing of ethanol concentration, the more nanosilver and probe molecules were oxidized, resulting in SERS intensity decreasing at 1615 cm−1. The decreased SERS intensity was linear to ethanol in the range of 0.008–0.40 mg/L, with a detection limit of 3 μg/L. This method was applied to determination of ethanol in beer and serum samples, with satisfactory results.