Combined reflectance spectroscopy and stochastic modeling approach for noninvasive hemoglobin determination via palpebral conjunctiva.
Combined reflectance spectroscopy and stochastic modeling approach for noninvasive hemoglobin determination via palpebral conjunctiva.
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DOI：
10.1002/phy2.192
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Alber, Mark
中科院分区：
文献类型：
--
作者：
Kim, Oleg;McMurdy, John;Jay, Gregory;Lines, Collin;Crawford, Gregory;Alber, Mark
将光子在多层眼睑组织中的随机传播模型与反射光谱法相结合，研究了眼睑结膜反射光谱法测定血红蛋白（Hgb）。所开发的模型是第一个生物相关的眼睑组织模型，这被证明是非常好的近似测量光谱。组织光学参数的定义使用以前的组织学和显微镜研究的人眼睑。在校正模型参数后，计算了反射光谱对血红蛋白水平和血氧变化的响应。正常和低血红蛋白水平的成人受激反射光谱同意与实验数据的血红蛋白浓度从8.1至16.7克/分升。将提取的Hgb水平与体外Hgb测量值进行比较。交叉验证的均方根误差为1.64 g/dL。结果表明，该方法对临床诊断的贫血病例的敏感性估计为86%。该模型与光谱测量的结合为人类结膜的非侵入性研究提供了一种新的工具，以帮助诊断血液疾病，如贫血。我们的研究描述了一种新的方法相结合的随机建模和实验光谱，以解决一个重要的生物医学问题的非侵入性血红蛋白评估通过眼睑结膜。开发的模型是第一个生物相关的详细表示光传播通过眼睑组织，这被证明是提供非常好的预测测量光谱。
A combination of stochastic photon propagation model in a multilayered human eyelid tissue and reflectance spectroscopy was used to study palpebral conjunctiva spectral reflectance for hemoglobin (Hgb) determination. The developed model is the first biologically relevant model of eyelid tissue, which was shown to provide very good approximation to the measured spectra. Tissue optical parameters were defined using previous histological and microscopy studies of a human eyelid. After calibration of the model parameters the responses of reflectance spectra to Hgb level and blood oxygenation variations were calculated. The stimulated reflectance spectra in adults with normal and low Hgb levels agreed well with experimental data for Hgb concentrations from 8.1 to 16.7 g/dL. The extracted Hgb levels were compared with in vitro Hgb measurements. The root mean square error of cross‐validation was 1.64 g/dL. The method was shown to provide 86% sensitivity estimates for clinically diagnosed anemia cases. A combination of the model with spectroscopy measurements provides a new tool for noninvasive study of human conjunctiva to aid in diagnosing blood disorders such as anemia. Our study describes a novel method combining stochastic modeling and experimental spectroscopy to address an important biomedical problem of noninvasive hemoglobin assessment via palpebral conjunctiva. The developed model is the first biologically relevant detailed representation of light propagation through eyelid tissue, which was shown to provide very good predictions for the measured spectra.