Establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (KKU-100)
Establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (KKU-100)
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DOI：
10.3748/wjg.v11.i22.3392
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发表时间：
2005-06-14
影响因子：
4.3
通讯作者：
Miwa, Masanao
中科院分区：
文献类型：
--
作者：
Sripa, Banchob;Leungwattanawanit, Saman;Miwa, Masanao
目的：建立一种新的肝后睾吸虫胆管癌细胞系。方法：新鲜的肝活检和胆汁标本来自一位65岁的泰国女性肝门部胆管癌患者。消化后，在Ham’s F12培养基中培养细胞。然后对建立的细胞系进行生长动力学、细胞形态、免疫细胞化学和细胞遗传学表征。结果：该细胞系的原发瘤为低分化管状腺癌。胆汁检查显示恶性细胞，O。灵猫卵胆管癌细胞系KKU-100在原代培养后4个月建立，群体倍增时间为72 h。KKU-100具有致密的杯状上皮细胞。免疫细胞化学，该细胞系表现出细胞角蛋白，EMA，CEA，和CA 125，但不是甲胎蛋白（AFP），CA 19 -9，结蛋白，c-met，或p53。这种蛋白质表达与原发性肿瘤的蛋白质表达平行。细胞遗传学分析确定了非整倍体核型与模态染色体数为78和显着的染色体结构变化。KKU-100细胞接种到裸鼠产生了可移植的，低分化腺癌，类似于原始tumor.CONCLUSION：KKU-100是第一个鸡蛋证明，后睾吸虫相关的胆管癌细胞系，这应该被证明是有用的，为进一步调查这种癌症的肿瘤生物学。(C)2005年WJG出版社和Elsevier Inc. All rights reserved.
AIM: To establish and characterize a new cholangiocarcinoma cell line from a patient living in the Opisthorchis viverrini (O. viverrini) endemic area of Northeast Thailand.METHODS: Fresh liver biopsy and bile specimens were obtained from a 65-year-old Thai woman with cholangiocarcinoma of the porta hepatis. After digestion, the cells were cultured in Ham's F12 media. The established cell line was then characterized for growth kinetics, cell morphology, immunocytochemistry and cytogenetics. Tumorigenicity of the cell line was determined by heterotransplanting in nude mice.RESULTS: The primary tumor was a poorly differentiated tubular adenocarcinoma. Examination of the bile revealed malignant cells with O. viverrini eggs. The cholangiocarcinoma cell line KKU-100 was established 4 mo after the primary culture-population doubling time was 72 h. KKU-100 possesses compact and polygonal-shaped epithelial cells. Immunocytochemically, this cell line exhibited cytokeratin, EMA, CEA, and CA125, but not alpha-fetoprotein (AFP), CA19-9, desmin, c-met, or p53. Such protein expressions parallel those of the primary tumor. Cytogenetic analysis identified aneuploidy karyotypes with a modal chromosome number of 78 and marked chromosomal structural changes. Inoculation of KKU-100 cells into nude mice produced a transplantable, poorly differentiated aden-ocarcinoma, similar to the original tumor.CONCLUSION: KKU-100 is the first egg-proven, Opisthorchis-associated cholangiocarcinoma cell line, which should prove useful for further investigations of the tumor biology of this cancer. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.