Medium-scale preparation of useful metabolites of aromatic compounds via whole-cell fermentation with recombinant organisms
Medium-scale preparation of useful metabolites of aromatic compounds via whole-cell fermentation with recombinant organisms
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DOI：
10.1021/op020013s
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发表时间：
2002-07-01
影响因子：
3.4
通讯作者：
Hudlicky, T
中科院分区：
文献类型：
--
作者：
Endoma, MA;Bui, VP;Hudlicky, T
用大肠杆菌JM 109（pDTG 601）在中等规模（10-15 L）上全细胞发酵芳香化合物产生对映体纯的环己二烯二醇。描述了发酵的详细程序，并提供了几种代谢产物的产率。使用E. coliJM 109（pDTG 602）中获得儿茶酚类化合物。二烯二醇可用于不对称合成，并列出了源自这些代谢物的几个重要靶标。
The whole-cell fermentation of aromatic coumpounds with Escherichia coli JM109 (pDTG601) on a medium scale (10-15 L) produces enantiopure cyclohexadienediols. A detailed procedure for the fermentation is described, and yields for several metabolites are provided. A similar procedure using E. coli JM109 (pDTG602) affords catechols. The dienediols are useful for asymmetric synthesis, and several important targets originating from these metabolites are tabulated.