Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells.
Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells.
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高等真核生物染色体间相互作用的发现指出了基因组结构和基因表达之间的功能相互作用，挑战了转录作为一维过程的观点。然而，染色体间相互作用的程度和潜在的机制是未知的。在这里，我们提出了第一个全基因组的转录相互作用的分析使用小鼠珠蛋白基因在红细胞组织。我们的研究结果表明，活跃的珠蛋白基因与数百个其他转录基因，揭示了广泛的和优惠的内和染色体间的转录interactomes。我们发现，转录因子Klf1介导的Klf1调节的基因在有限数量的专门的转录工厂的优先共协会。我们的研究结果建立了一个新的基因表达模式，这意味着积极的共调控基因和它们的调控因子合作，创造专门的核热点优化有效和协调的转录控制。
The discovery of interchromosomal interactions in higher eukaryotes points to a functional interplay between genome architecture and gene expression, challenging the view of transcription as a one-dimensional process. However, the extent of interchromosomal interactions and the underlying mechanisms are unknown. Here we present the first genome-wide analysis of transcriptional interactions using the mouse globin genes in erythroid tissues. Our results show that the active globin genes associate with hundreds of other transcribed genes, revealing extensive and preferential intra- and interchromosomal transcription interactomes. We show that the transcription factor Klf1 mediates preferential co-associations of Klf1-regulated genes at a limited number of specialized transcription factories. Our results establish a new gene expression paradigm, implying that active co-regulated genes and their regulatory factors cooperate to create specialized nuclear hot spots optimized for efficient and coordinated transcriptional control.