Enhancement of cordyceps polysaccharide production via biosynthetic pathway analysis in Hirsutella sinensis
Enhancement of cordyceps polysaccharide production via biosynthetic pathway analysis in Hirsutella sinensis
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DOI：
10.1016/j.ijbiomac.2016.08.002
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发表时间：
2016-11-01
影响因子：
8.2
通讯作者：
Zheng, Yu-Guo
中科院分区：
文献类型：
--
作者：
Lin, Shan;Liu, Zhi-Qiang;Zheng, Yu-Guo
研究了不同浓度硫酸盐对H.对中华白海豚进行了调查，发现硫酸锰的效果最好。在0 d（d）添加2 mM硫酸锰为最佳添加条件，CP产量达到最佳，为5.33%，比对照提高了93.3%。此外，在两种条件下的培养过程中，三种主要的CP前体的消耗进行了研究。培养6d，胞内甘露糖含量下降43.1%，与CP积累速率急剧增加相对应，甘露糖是CP产生的最佳前体物质。随后，在H.研究了CP生物合成的重要组成部分-中国对虾的CP合成基因及其转录水平。cpsA基因转录水平显著上调5.35倍，是甘露糖和CP合成的关键基因。本研究表明，添加硫酸锰是提高CP产量的一种有效而简单的方法。基于生物合成途径的转录分析有助于发现关键基因，更好地理解CP生物合成。(C)© 2016 Elsevier B. V.版权所有。
The addition of various sulfates for enhanced cordyceps polysaccharide (CP) production in submerged cultivation of H. sinensis was investigated, and manganese sulfate was found the most effective. 2 mM of manganese sulfate on 0 day (d) was investigated as the optimal adding condition, and the CP production reached optimum with 5.33%, increasing by 93.3% compared with the control. Furthermore, the consumption of three main precursors of CP was studied over cultivation under two conditions. Intracellular mannose content decreased by 43.1% throughout 6 days cultivation, which corresponded to CP accumulation rate sharply increased from 0 d to 6 d, and mannose was considered as the most preferred precursor for generating CP. Subsequently, mannose biosynthetic pathway was constructed and verified for the first time in H. sinensis, which constituted the important part of CP biosynthesis, and transcriptional levels of the biosynthetic genes were studied. Transcriptional level of gene cpsA was significantly up-regulated 5.35-fold and it was a key gene involved both in mannose and CP biosynthesis. This study demonstrated that manganese sulfate addition is an efficient and simple way to improve CP production. Transcriptional analysis based on biosynthetic pathway was helpful to find key genes and better understand CP biosynthesis. (C) 2016 Elsevier B.V. All rights reserved.