Molecular cloning and expression analysis of interleukin-1β and interleukin-1 receptor type I genes in yellow catfish (Pelteobagrus fulvidraco): Responses to challenge of Edwardsiella ictaluri
Molecular cloning and expression analysis of interleukin-1β and interleukin-1 receptor type I genes in yellow catfish (Pelteobagrus fulvidraco): Responses to challenge of Edwardsiella ictaluri
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黄颡鱼 (Pelteobagrus fulvidraco) 中白细胞介素 1 β 和白细胞介素 1 受体 I 型基因的分子克隆和表达分析：对鲶鱼爱德华氏菌攻击的反应
DOI：
10.1016/j.cbpb.2018.05.001
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发表时间：
2018-09-01
影响因子：
2.2
通讯作者：
Wei, Kai-Jian
中科院分区：
文献类型：
--
作者：
Liao, Chen-Lei;Zhang, Gui-Rong;Wei, Kai-Jian
白介素1β(IL-1β)是一种关键的早期促炎细胞因子，在调节免疫反应和诱导一系列炎症反应中发挥重要作用。白介素1I型受体(IL-1RI)是一种IL-1β受体，可介导IL-1依赖的激活。本研究克隆了黄Web鱼(Pelteobagus Fulvidraco)Pf_IL-1β和Pf_IL-1RI基因的部分cDNA序列。PF_IL-1β和pF_IL-1RI基因的开放阅读框(ORF)分别编码280和543个氨基酸。这两个基因推导的氨基酸序列与其他硬骨鱼的氨基酸序列具有高度保守的结构。实时荧光定量聚合酶链式反应结果显示，PF_IL-1β在干肾和血液中的表达水平较高，PF_IL-1RI在血液中的表达水平较高，在肝脏中的表达水平较高。个体发育表达分析表明，pF_IL-1β和pF_IL-1RI基因可能在胚胎发育过程中发挥重要作用。在爱德华氏菌攻击后，躯干肾、头肾、血液、脾、心脏和肝脏中PF_IL-1β和PF_IL-1RI基因的mRNA表达水平均上调。Western印迹分析表明，PF_IL-1β蛋白在脾和头肾中高表达，而在成年个体鳍中不表达。这些结果表明，Pf_IL-1β和Pf_IL-1RI基因可能在黄点叉尾轮虫的免疫调节和防御中发挥重要作用。
Interleukin-1 beta (IL-1 beta) is one of the pivotal early pro-inflammatory cytokines, which play important roles in regulating immune response and inducing a series of inflammatory reactions to infections. Interleukin-1 type I receptor (IL-1RI) is a receptor of the IL-1 beta that can mediate IL-1-dependent activation. In this study, partial cDNA sequences of the Pf_IL-1 beta and Pf_IL-1RI genes were cloned from yellow catfish (Pelteobagrus fulvidraco). The open reading frames (ORF) of Pf_IL-1 beta and Pf_IL-1RI genes encode putative peptides of 280 and 543 amino acids, respectively. The deduced amino acid sequences of these two genes shared highly conserved structures with those from other teleosts. Quantitative real-time PCR results showed that the pf_IL-1 beta mRNA had relatively high expression levels in trunk kidney and blood, and the Pf_IL-1RI mRNA was highly expressed in blood and had relatively high expression level in liver. Ontogenetic expression analyses indicate that the Pf_IL-1 beta and Pf_IL-1RI genes may play important roles during the embryonic developmental stages. The mRNA expression levels of Pf_IL-1 beta and Pf_IL-1RI genes were up-regulated in the trunk kidney, head kidney, blood, spleen, heart and liver after Edwardsiella ictaluri challenge. Western blot analyses showed that Pf_IL-1 beta protein was highly expressed in the spleen and head kidney, but not in the fin of adult individuals. These results suggest that the Pf_IL-1 beta and Pf_IL-1RI genes may play significant roles in the immune regulation and defense against E. ictaluri in the yellow catfish.