The effect of orientation within a chimeric peptide on the immunogenicity of Chlamydia trachomatis epitopes.
The effect of orientation within a chimeric peptide on the immunogenicity of Chlamydia trachomatis epitopes.
复制标题
嵌合肽内的方向对沙眼衣原体表位免疫原性的影响。
DOI：
10.1016/0161-5890(95)00157-3
复制
发表时间：
1996
影响因子：
3.6
通讯作者：
delaMaza,LM
中科院分区：
文献类型：
--
作者：
Peterson,EM;Cheng,X;Qu,Z;delaMaza,LM
代表沙眼衣原体主要外膜蛋白可变结构域（VD）1和4的血清型C和E，分别已被证明在小鼠中引起中和抗体反应。为了评估嵌合肽内的位置是否影响表位的免疫原性，制备了两种构建体VD 1-4和VD 4-1，其中VD相对于氨基和羧基末端的位置被旋转。在第0天用在完全弗氏佐剂（FA）中的100 μg肽免疫C57 BL 10小鼠，然后在第14天用在不完全FA中的肽（100 μg）免疫。到第21天，通过ELISA测量的两种嵌合体中的免疫显性表位是位于羧基末端的表位。VD 1-4抗血清的pepscan显示VD 4中的主峰，其先前已通过中和MAbs鉴定。VD 4-1抗血清在VD 1区产生一个峰，该峰与先前用中和MAb定位的区域不一致。VD 1-4抗血清能中和E型血清，而VD 4-1抗血清不能.总之，嵌合肽内这些衣原体表位的位置极大地影响了所得的免疫应答。
Peptides representing the Chlamydia trachomatis major outer membrane protein variable domains (VD) 1 and 4 of serovars C and E, respectively, have been shown to elicit a neutralizing antibody response in mice. To assess whether the position within a chimeric peptide influences the immunogenicity of the epitopes, two constructs, VD 1–4 and VD 4-1, were made in which the position of the VD relative to the amino and carboxy terminals were rotated. C57BL 10 mice were immunized with 100 μg of peptide in complete Freund's adjuvant (FA) on day 0, followed by an immunization with peptide (100 μg) in incomplete FA on day 14. By day 21 the immunodominant epitope in both chimeras as measured by ELISA was the one located at the carboxy terminus. A pepscan of the VD 1–4 antisera revealed a main peak in VD 4 which had been previously identified by neutralizing MAbs. The VD 4-1 antisera gave a peak in the VD 1 region which did not correspond to regions previously mapped with neutralizing MAbs. The VD 1–4 antisera but not the VD 4-1 antisera was able to neutralize in vitro serovar E. In summary, the position of these chlamydial epitopes within a chimeric peptide greatly influenced the resulting immune response.