Effect of ocular hypertension on retinal GABAergic activity
Effect of ocular hypertension on retinal GABAergic activity
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DOI：
10.1016/j.neuint.2007.08.014
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发表时间：
2008-03-01
影响因子：
4.2
通讯作者：
Rosenstein, Ruth E.
中科院分区：
文献类型：
--
作者：
Moreno, Maria Cecilia;de Zavalia, Nuria;Rosenstein, Ruth E.
谷氨酸和γ-氨基丁酸（GABA）是主要的兴奋性和抑制性视网膜神经递质。这些信号之间的平衡是视网膜水平组织的关键原则。虽然谷氨酸诱导的兴奋性毒性可介导青光眼视网膜神经节细胞死亡，但GABA能系统以前未在这种疾病中进行过研究。本工作的目的是研究透明质酸（HA）诱导的高眼压眼的视网膜GABA能活性。为了这个目的，每周注射HA单侧在大鼠前房，而对侧眼注射生理盐水溶液。在3周的治疗与HA，GABA周转率，谷氨酸脱羧酶活性，谷氨酸和高K+诱导的GABA释放显着下降，而GABA的摄取增加HA治疗的眼睛。与注射溶剂的眼睛相比，注射HA的眼睛中叔丁基双环硫代磷酸酯（TBPS）与GABA（A）/苯二氮卓类Cl-通道的结合显著增加。GABA摄取和TBPS结合的变化持续6周的治疗与HA。这些结果表明，视网膜GABA能活性的功能障碍，在高血压的眼睛，这可能表明GABA参与青光眼性神经病变。(C)2007爱思唯尔有限公司保留所有权利。
Glutamate and gamma-aminobutyric acid (GABA) are major excitatory and inhibitory retinal neurotransmitters. The balance between these signals is a key principle of organization at retinal level. Although glutamate-induced excitotoxicity could mediate retinal ganglion cell death in glaucoma, the GABAergic system was not previously examined in this disease. The aim of this work was to study the retinal GABAergic activity in eyes with ocular hypertension induced by hyaluronic acid (HA). For this purpose, weekly injections of HA were performed unilaterally in the rat anterior chamber, whereas the contralateral eye was injected with saline solution. At 3 weeks of treatment with HA, GABA turnover rate, glutamic acid decarboxylase activity, and both glutamate- and high K+-induced GABA release significantly decreased, whereas GABA uptake increased in HA-treated eyes. The binding of t-butylbicyclophosphorothionate (TBPS) to GABA(A)/benzodiazepine Cl- channels significantly increased in eyes injected with HA as compared with vehicle-injected eyes. Changes in GABA uptake and TBPS binding persisted at 6 weeks of treatment with HA. These results indicate a dysfunction of the retinal GABAergic activity in hypertensive eyes, which could suggest the involvement of GABA in glaucomatous neuropathy. (C) 2007 Elsevier Ltd. All rights reserved.