Electron microscope characteristics of dentin repair after hydroxylapatite direct pulp capping in rats.
Electron microscope characteristics of dentin repair after hydroxylapatite direct pulp capping in rats.
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大鼠羟基磷灰石直接盖髓后牙本质修复的电镜特征
DOI：
10.1111/j.1600-0714.1991.tb00413.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
W. B. Donohue
中科院分区：
文献类型：
--
作者：
L. Jaber;C. Mascrès;W. B. Donohue
为研究羟基磷灰石（HA）对牙髓的成骨作用，设计了大鼠上磨牙盖髓实验。在全身麻醉下，14只雄性Sprague-Dawley大鼠的磨牙用Osteogen（HA）或Dycal作为对照材料盖髓。盖髓后，用银汞合金修复上颌磨牙的洞，调整并在上颌两侧的磨牙上密封一个儿科钢冠。7天后，用Dycal处理的牙髓中坏死和急性炎症的区域比用Osteogen处理的更明显。硬组织形成开始出现在牙髓中的牙本质碎片周围，并从腔壁延伸到牙髓中，而不管所使用的材料。此外，当使用Osteogen时，这种钙化物质分散在整个牙髓中，但在Dycal处理的牙髓中没有观察到。硬组织的形成被认为是由于牙髓中发现的假定的成纤维细胞和成牙本质细胞。28天后，当使用Dycal时，观察到致密的牙本质组织桥接暴露部位。成骨素形成的牙本质组织多为球形，分布不规则。由于Osteogen倾向于在牙髓中引起营养不良性钙化区域，因此不建议将其用于人类的盖髓目的，因为这些钙化区域会使未来的牙髓治疗变得困难。
In order to study the osteogenic action of hydroxylapatite (HA) on the dental pulp, a pulp capping experiment was designed using the rat upper molar. Under general anesthesia, molar teeth in 14 male Sprague-Dawley rats were pulp capped with Osteogen (HA) or with Dycal as a control material. After pulp capping, the maxillary molars cavities were restored with amalgam and a pedodontic steel crown was adjusted and sealed over the molar teeth on either side of the maxilla. After 7 days, the areas of necrosis and acute inflammation were more evident in the pulps treated with Dycal than with Osteogen. Hard tissue formation began to appear around dentinal chips in the pulp and extended from the cavity walls into the pulp regardless of the material that was used. Furthermore, this calcified material was scattered throughout the pulp when Osteogen was used, but was not observed in the Dycal treated pulps. The hard tissue formation was thought to be due to the putative fibroblasts and odontoblasts found in the pulp. After 28 days dense dentinal tissue was observed bridging the exposure site when Dycal was used. The dentinal tissues formed with Osteogen was always of a globular type, and showed an irregular distribution. Since Osteogen tends to cause areas of dystrophic calcification in the pulp, its use is not be recommended for pulp capping purposes in humans, because these areas of calcification would make future endodontic treatment difficult.