Diet-induced obesity impairs spermatogenesis: a potential role for autophagy.
Diet-induced obesity impairs spermatogenesis: a potential role for autophagy.
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饮食引起的肥胖损害精子发生：自噬的潜在作用
DOI：
10.1038/srep43475
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发表时间：
2017-03-09
影响因子：
4.6
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Mu Y;Yan WJ;Yin TL;Zhang Y;Li J;Yang J
自噬是一个进化保守的过程，在维持一系列细胞功能中起着至关重要的作用。研究发现，自噬密切参与精子发生的生理过程，调控精子的存活和运动。然而，自噬在高脂肪饮食（HFD）诱导的精子发生受损中的作用仍不清楚。本研究旨在探讨自噬在hfd诱导的精子发生缺陷中的作用，采用氯喹（CQ）抑制自噬，雷帕霉素（RAP）诱导自噬。3-甲基腺嘌呤（3-MA）和CQ通过睾丸内注射给药。研究了CQ和3-MA对与棕榈酸（PA）共培养精子参数的影响。我们还收集了肥胖、不育男性患者的精液样本，以检测自噬水平。结果表明，经CQ处理的HFD小鼠精子发生改善。用CQ抑制自噬可以改善HFD雄性小鼠的生育能力。体外实验结果表明，CQ和3-MA均能抑制HFD或PA治疗引起的精子病理改变。此外，在肥胖、不育男性患者的精子样本中也观察到过度的自噬激活。
Autophagy is an evolutionarily conserved process that plays a crucial role in maintaining a series of cellular functions. It has been found that autophagy is closely involved in the physiological process of spermatogenesis and the regulation of sperm survival and motility. However, the role of autophagy in high-fat diet (HFD)-induced impaired spermatogenesis remains unknown. This study was designed to investigate the role of autophagy in HFD-induced spermatogenesis deficiency and employed chloroquine (CQ) to inhibit autophagy and rapamycin (RAP) to induce autophagy. 3-methyladenine (3-MA) and CQ were administered via intratesticular injectionin vivo. The effects of CQ and 3-MA on the parameters of spermatozoa co-cultured with palmitic acid (PA)in vitrowere also investigated. Human semen samples from obese, subfertile male patients were also collected to examine the level of autophagy. The results suggested that HFD mice subjected to CQ showed improved spermatogenesis. Inhibiting autophagy with CQ improved the decreased fertility of HFD male mice. Moreover, thein vivoandin vitroresults indicated that both CQ and 3-MA could suppress the pathological changes in spermatozoa caused by HFD or PA treatment. Additionally, the excessive activation of autophagy was also observed in sperm samples from obese, subfertile male patients.