Neuroprotective effects of the Chinese Yi-Qi-Bu-Shen recipe extract on injury of rat hippocampal neurons induced by hypoxia/reoxygenation.
Neuroprotective effects of the Chinese Yi-Qi-Bu-Shen recipe extract on injury of rat hippocampal neurons induced by hypoxia/reoxygenation.
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DOI：
10.1016/j.jep.2012.10.046
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发表时间：
2013-01
影响因子：
5.4
通讯作者：
Deshan Liu;Yong-hong Zhou;Er-shun Liang;Wei Li;Wei-wei Lin;Fei-Fei Chen-Fei;Wei Gao
中科院分区：
文献类型：
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作者：
Deshan Liu;Yong-hong Zhou;Er-shun Liang;Wei Li;Wei-wei Lin;Fei-Fei Chen-Fei;Wei Gao
目的探讨益气补肾方通过降血糖、抗氧化作用改善2型糖尿病大鼠脑功能，对缺氧复氧所致神经元损伤的保护作用，并探讨其作用机制。第10天，将原代培养的海马神经元随机分为5组：正常组(NG)、缺氧/复氧组(HG)和YB小、中、大剂量保护组(分别为SG、MG和LG)。YB保护组在复氧前给予不同浓度的YB含药血清。用试剂盒测定四甲基偶氮唑盐代谢率、丙二醛含量、超氧化物歧化酶和乳酸脱氢酶活性。用流式细胞仪检测海马神经元凋亡率。结果HG组细胞超氧化物歧化酶活性、细胞存活率、bcl2/bax比值、bcl2mRNA表达明显低于正常对照组(均P<0.01)，而丙二醛、乳酸脱氢酶含量、细胞凋亡率及Bax mRNA表达明显高于正常对照组(均P<0.01)。与HG组比较，YB各剂量组大鼠海马神经元的超氧化物歧化酶活性、细胞存活率、bcl2mRNA表达及bcl2/bax比值均显著升高(P均<0.01)，而丙二醛和乳酸脱氢酶水平、细胞凋亡率及Bax mRNA表达均显著降低(P均<0.01，P<0.05)，且呈剂量依赖关系。结论YB提取物对缺氧/复氧所致海马神经元损伤具有保护作用，其机制可能与其抗氧化活性和细胞凋亡调控有关。
OBJECTIVETo explore the protective effects of the Chinese Yi-Qi-Bu-Shen recipe (YB) against neuronal injury induced by hypoxia–reoxygenation, which has shown beneficial effect in improving the brain function of type 2 diabetics likely through its antihyperglycemic, antioxidant activity, and investigate its mechanisms.METHODSThe bilateral hippocampus was collected from newborn rats to establish single cell suspension. On the 10th day, the primarily cultured hippocampal neurons were randomly divided into five groups: the normal group (NG), the hypoxia/reoxygenation group (HG), and groups protected with small, medium and large dosages of YB (SG, MG and LG, respectively). The YB-protected groups were treated with different concentrations of YB containing serum before reoxygenation. The metabolic rate of MTT, the malondialdehyde (MDA) content, and the activity of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were measured with assay kits. The apoptosis rate of hippocampal neurons were tested using flow cytometry analysis. RT-PCR was used to evaluate the mRNA expressions of bcl-2 and bax genes.RESULTSThe SOD activity, the cell survival rate, the bcl-2/bax ratio, and the bcl-2mRNA expression in the HG group were significantly lower (all P<0.01), but the levels of MDA and LDH, the apoptosis rate, and the bax mRNA expression were higher (all P<0.01) than those in the NG group. The SOD activity, the cell survival, the bcl-2mRNA expression, and the bcl-2/bax ratio were significantly higher in all of the YB-protected groups (all P<0.01), but the level of MDA and LDH, the apoptosis rate, and the bax mRNA expression were lower (P<0.01, P<0.05) than those in the HG group in a dose dependent manner.CONCLUSIONThe YB extract has a protective effect on hippocampal neurons against injury induced by hypoxia/reoxygenation, through its antioxidant activity and the regulation of apoptosis.