Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius.
Molecular epidemiology and population structure of the honey bee brood pathogen Melissococcus plutonius.
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DOI：
10.1038/ismej.2014.20
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Haynes EG
中科院分区：
文献类型：
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作者：
Budge GE;Shirley MD;Jones B;Quill E;Tomkies V;Feil EJ;Brown MA;Haynes EG
欧洲蜜蜂污巢病是一种严重危害欧洲蜜蜂的巢病，其病原是雨生蜜蜂蜜球菌（Melissococcus plutonius）。EFB仍然是一个威胁，因为对疾病流行病学的理解不足。我们使用最近发表的多位点序列分型方法来检测206 M。plutonius分离物从英格兰和威尔士的爆发中恢复了2年。我们检测到15种不同的序列类型（ST），通过eBURST和系统发育分析将其解析为三个克隆复合体（CC）3、12和13。CC 3内的单和双位点变异是最丰富和最广泛的基因型，占病例的85%。相比之下，CC 12和13则较为罕见，主要发现于采样密度较高的地理区域，这与最近的引入和局部传播相一致。K-函数分析和点间距离检验显示，显着的地理聚集在5个常见的ST，但ST之间的扩散模式不同。我们注意到CC的致病性似乎各不相同，并且由致病性更强的变体引起的感染更可能导致蜜蜂群体的破坏，而不是治疗。这些研究结果的重要性，以提高我们对疾病的病因学和控制的理解进行了讨论。
Melissococcus plutonius is the causative agent of European foulbrood (EFB), which is a serious brood disease of the European honey bee (Apis mellifera). EFB remains a threat because of a poor understanding of disease epidemiology. We used a recently published multi-locus sequence typing method to characterise 206 M. plutonius isolates recovered from outbreaks in England and Wales over the course of 2 years. We detected 15 different sequence types (STs), which were resolved by eBURST and phylogenetic analysis into three clonal complexes (CCs) 3, 12 and 13. Single and double locus variants within CC3 were the most abundant and widespread genotypes, accounting for 85% of the cases. In contrast, CCs 12 and 13 were rarer and predominantly found in geographical regions of high sampling intensity, consistent with a more recent introduction and localised spread. K-function analysis and interpoint distance tests revealed significant geographical clustering in five common STs, but pointed to different dispersal patterns between STs. We noted that CCs appeared to vary in pathogenicity and that infection caused by the more pathogenic variants is more likely to lead to honey bee colony destruction, as opposed to treatment. The importance of these findings for improving our understanding of disease aetiology and control are discussed.