Adult-born hippocampal neurons mature into activity-dependent responsiveness
Adult-born hippocampal neurons mature into activity-dependent responsiveness
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DOI：
10.1111/j.1460-9568.2003.02986.x
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发表时间：
2003-11-01
影响因子：
3.4
通讯作者：
Kempermann, G
中科院分区：
文献类型：
--
作者：
Jessberger, S;Kempermann, G
哺乳动物的海马体是一种高度可塑性的大脑结构，在整个成年期都会产生新的神经元。在学习任务中挑战海马体后，这些新神经元是否定性和定量地参与活动依赖性基因表达？成年小鼠被注射溴脱氧尿苷（BrdU）来标记分裂细胞，事实上，在莫里斯水迷宫任务的采集阶段，单个BrdU标记的新神经元表达立即早期基因蛋白（IEGp）c-fos，其程度与较老的颗粒细胞相同。为了评估所有新神经元的反应性，我们还分析了红藻氨酸 (KA) 或戊四氮 (PTZ) 诱导的癫痫发作后三种 IEGps（c-fos、zif268 和 Homer1A）的表达模式。我们发现，在 2 至 5 周的成熟期后，成体产生的颗粒细胞参与 IEGp 表达的比例与老年颗粒细胞群中的比例相当。因此，新的神经元似乎定量地整合到海马回路中，这表明对海马功能的相关贡献。
The mammalian hippocampus is a highly plastic brain structure in which new neurons are generated throughout adulthood. Do these new neurons qualitatively and quantitatively participate in the activity-dependent gene expression after challenging the hippocampus in a learning task? Adult mice were injected with bromodeoxyuridine (BrdU) to label dividing cells and indeed, individual BrdU-labelled new neurons expressed the immediate early gene protein (IEGp) c-fos during the acquisition phase of the Morris water maze task to the same extend as older granule cells. To assess the responsiveness of all new neurons we also analysed the expression patterns of three IEGps (c-fos, zif268 and Homer1A) after kainic acid (KA)- or pentylenetetrazol (PTZ)-induced seizures. We found that after a maturation period of between 2 and 5 weeks a comparable ratio of adult-generated granule cells participated in IEGp-expression as in the population of older granule cells. Thus, new neurons appear quantitatively integrated into hippocampal circuits, suggestive of a relevant contribution to hippocampal function.