Relation between stable isotope ratios in human red blood cells and hair: implications for using the nitrogen isotope ratio of hair as a biomarker of eicosapentaenoic acid and docosahexaenoic acid
Relation between stable isotope ratios in human red blood cells and hair: implications for using the nitrogen isotope ratio of hair as a biomarker of eicosapentaenoic acid and docosahexaenoic acid
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DOI：
10.3945/ajcn.2009.28482
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发表时间：
2009-12-01
影响因子：
7.1
通讯作者：
O'Brien, Diane M.
中科院分区：
文献类型：
--
作者：
Nash, Sarah H.;Kristal, Alan R.;O'Brien, Diane M.
背景资料：在爱斯基摩人中，红细胞（RBC）的氮同位素比率（表示为Δ N-15）与RBC长链ω-3（n-3）脂肪酸二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）高度相关。由于Δ N-15也可以在头发样本中测量，它可以提供一个非侵入性的，回顾性的生物标志物EPA和DHA intakes.Objectives：我们调查了头发和红细胞中的Δ N-15之间的一致性，然后评估头发Δ N-15和红细胞EPA和DHA之间的关系。我们还评估了碳同位素比率的一致性（delta C-13）之间的头发和红细胞，因为delta C-13已被提出作为饮食生物标志物在其他人群。设计：我们在144名爱斯基摩人的社区样本中评估了头发和RBC δ N-15和δ C-13之间的关系，并检查了δ N-15和δ C-13之间的相关性。结果：我们发现头发与RBC δ N-15（r = 0.91）和δ C-13（r = 0.87）之间具有1：1的良好一致性。头发的同位素比率比红细胞的比率高出1.5ppm的δ N-15和2.3ppm的δ C-13。头发δ N-15与红细胞EPA和DHA之间有很强的相关性（r = 0.83和0.84，respectively）。结论：这些结果支持使用头发δ N-15值作为EPA和DHA摄入量的生物标志物。由于毛发采集是非侵入性的，样本不需要特殊处理，因此在大规模人群中进行EPA和DHA摄入量的研究可以使用生物标志物而不是自我报告来评估这些脂肪酸。美国临床营养杂志2009;90：1642-7.
Background: The nitrogen isotope ratio (expressed as delta N-15) of red blood cells (RBCs) is highly correlated with the RBC long-chain omega-3 (n-3) fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in Yup'ik Eskimos. Because delta N-15 can also be measured in hair samples, it could provide a noninvasive, retrospective biomarker for EPA and DHA intakes.Objectives: We investigated the agreement between delta N-15 in hair and RBCs and then evaluated the relations between hair delta N-15 and RBC EPA and DHA. We also assessed the agreement in carbon isotope ratios (delta C-13) between hair and RBCs, because delta C-13 has been proposed as a dietary biomarker in other populations.Design: We assessed relations between hair and RBC delta N-15 and delta C-13 in a community-based sample of 144 Yup'ik Eskimos and examined the correlations between delta N-15 and RBC EPA and DHA in a subset of these participants (n = 44).Results: We showed a 1: 1 relation with good agreement between hair and RBC delta N-15 (r = 0.91) and delta C-13 (r = 0.87). Hair isotope ratios were greater than RBC ratios by 1.5 parts per thousand for delta N-15 and by 2.3 parts per thousand for delta C-13. There were strong correlations between hair delta N-15 and RBC EPA and DHA (r = 0.83 and 0.84, respectively).Conclusions: These results support the use of hair delta N-15 values as a biomarker of EPA and DHA intakes. Because hair collection is noninvasive and the samples require no special processing, studies of EPA and DHA intakes in large populations could use biomarkers rather than self-reports to assess these fatty acids. Am J Clin Nutr 2009;90:1642-7.