Stereo-Array Isotope Labeling Method for Studying Protein Structure and Dynamics
Stereo-Array Isotope Labeling Method for Studying Protein Structure and Dynamics
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DOI：
10.1007/978-94-007-4954-2_5
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发表时间：
2012-01-01
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影响因子：
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通讯作者：
Kainosho, Masatsune
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文献类型：
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作者：
Miyanoiri, Yohei;Takeda, Mitsuhiro;Kainosho, Masatsune
立体阵列同位素标记（SAIL）方法利用蛋白质的同位素标记模式优化，考虑到一个预期的核磁共振研究。SAIL蛋白是利用无细胞蛋白合成系统或细胞表达系统，将化学合成的氨基酸掺入目标蛋白中制备的。在过去的十年中，SAIL方法促进了各种各样的新研究，包括大蛋白质的高分辨率结构测定和蛋白质动力学研究。在本章中，介绍了与sail相关的方法的应用。
The stereo-array isotope labeling (SAIL) method utilizes proteins with isotope labeling patterns optimized with regard to an intended NMR study. The SAIL proteins are prepared by incorporating chemically synthesized amino acids into target proteins, using a cell-free protein synthesis system or a cellular expression system. Over the past decade, the SAIL method has been facilitating a wide variety of new investigations, including high-resolution structure determinations of large proteins and investigations of protein dynamics. In this chapter, the applications of SAIL-related approaches are introduced.