A pseudotype vesicular stomatitis virus containing Hantaan virus envelope glycoproteins G1 and G2 as an alternative to hantavirus vaccine in mice
A pseudotype vesicular stomatitis virus containing Hantaan virus envelope glycoproteins G1 and G2 as an alternative to hantavirus vaccine in mice
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DOI：
10.1016/j.vaccine.2005.12.040
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发表时间：
2006-04-05
期刊：
影响因子：
5.5
通讯作者：
Arikawa, J
中科院分区：
文献类型：
--
作者：
Lee, BH;Yoshimatsu, K;Arikawa, J
我们研究了携带汉坦病毒包膜糖蛋白（GPs）G1和G2（VSV Delta G*HTN）的水泡性口炎病毒（VSV）假型是否可以作为天然汉坦病毒的安全有效的替代品。用纯化的VSV Delta G*HTN颗粒免疫小鼠。在第二次免疫后，所有小鼠产生抗GP抗体，如在ELISA和中和试验中检测到的。第三次免疫后，用汉滩病毒攻击小鼠。在这些小鼠中既没有检测到抗NP抗体产生，也没有检测到汉滩病毒特异性CD8 T细胞反应。本研究证明了使用假型VSV系统作为开发汉坦病毒疫苗的工具的潜力。(c)2005爱思唯尔有限公司保留所有权利。
We examined whether a vesicular stomatitis virus (VSV) pseudotype bearing the hantavirus envelope glycoproteins (GPs) G1 and G2 (VSV Delta G*HTN) could be used as a safe and effective alternative to native hantavirus. Mice were immunized with purified particles of VSV Delta G*HTN. After the second immunization, all mice produced anti-GP antibody as detected in ELISA and a neutralization test. After the third immunization, the mice were challenged with Hantaan virus. Neither anti-NP antibody production nor Hantaan virus-specific CD8 T-cell reactions were detected in these mice. The present study demonstrated the potential of using a pseudotype VSV system as a tool for developing a hantavirus vaccine. (c) 2005 Elsevier Ltd. All rights reserved.