Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG, and two-photon microscopy.
Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG, and two-photon microscopy.
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DOI：
10.1364/boe.411620
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发表时间：
2021-02-01
影响因子：
3.4
通讯作者：
Richardson DJ
中科院分区：
文献类型：
--
作者：
Xu D;Liang S;Xu L;Bourdakos KN;Johnson P;Read J;Price JHV;Mahajan S;Richardson DJ
我们展示了一个连续波（CW）种子同步自由光参量放大器（OPA）泵浦皮秒，1 μm的激光，并显示其性能时，作为一个简单而强大的源无标记相干反斯托克斯拉曼散射（汽车），并发二次谐波产生（SHG），和双光子荧光显微镜epi-detection几何。平均功率水平超过175 mW，光谱分辨率为8 cm-1，脉冲持续时间为2 ps，非常适合生物科学和生物医学成像系统中的汽车显微镜。我们的OPA比传统用于汽车成像的“金标准”激光器和光学参量振荡器（OPO）组合或最近开发的飞秒脉冲泵浦的OPA系统简单得多[1]。通过改变极化的双极化锂酸盐（PPLN）OPA晶体的极化通道和温度以及OPA种子波长，实现了谐振之间的快速和准确的调谐。泵浦-斯托克斯频率失谐范围完全覆盖了用于脂质成像的C-H伸缩带。通过使三个多光子技术使用一个紧凑的，同步自由的激光源，我们的工作铺平了道路的翻译无标记的多光子显微镜成像从生物医学研究的成像为基础的诊断工具，用于医疗保健竞技场。
We demonstrate a continuous wave (CW) seeded synchronization-free optical parametric amplifier (OPA) pumped by a picosecond, 1 µm laser and show its performance when used as a simple yet powerful source for label-free coherent anti-Stokes Raman scattering (CARS), concurrent second harmonic generation (SHG), and two-photon fluorescence microscopy in an epi-detection geometry. The average power level of above 175 mW, spectral resolution of 8 cm−1, and 2 ps pulse duration are well optimized for CARS microscopy in bio-science and bio-medical imaging systems. Our OPA is a much simpler setup than either the “gold-standard” laser and optical parametric oscillator (OPO) combination traditionally used for CARS imaging, or the more recently developed OPA systems pumped with femtosecond pulses [1]. Rapid and accurate tuning between resonances was achieved by changing the poled channels and temperature of the periodically-poled lithium niobate (PPLN) OPA crystal together with the OPA seed wavelength. The Pump-Stokes frequency detuning range fully covered the C-H stretching band used for the imaging of lipids. By enabling three multiphoton techniques using a compact, synchronization free laser source, our work paves the way for the translation of label-free multi-photon microscopy imaging from biomedical research to an imaging based diagnostic tool for use in the healthcare arena.